Phenotypic Characterization of Peripheral Basophil Perturbations in the Antiphospholipid Syndrome

Benjamin Chaigne¹, Veronique Le Guern², Tali-Anne Szwebel¹, Romain Paule², Claire Le Jeunne³, Nathalie Costedoat-Chalumeau⁴ and Luc Mouthon⁵, ¹Service de Médecine Interne, Centre de Référence Maladies Systémiques Autoimmunes Rares d’Ile de France, hôpital Cochin, DHU Authors, Assistance Publique-Hôpitaux de Paris, Paris, France, ²Department of Internal Medicine, Department of Internal Medicine, Cochin University Hospital, Paris, France, ³Service de Médecine Interne, Hôpital Cochin, Centre de référence national pour les maladies systémiques autoimmunes rares d’Ile de France, DHU Authors, Assistance Publique-Hôpitaux de Paris (AP-HP), Paris, France, Paris, France, ⁴Service de médecine interne Pôle médecine, Hôpital Cochin, Centre de référence maladies auto-immunes et systémiques rares de l’île de France, Paris, France, ⁵Service de Médecine Interne, Hôpital Cochin, Centre de référence national pour les maladies systémiques autoimmunes rares d’Ile de France, DHU Authors, Assistance Publique-Hôpitaux de Paris (AP-HP), Paris, France ;Université Paris Descartes Sorbonne Paris, Paris, France

Meeting: 2017 ACR/ARHP Annual Meeting

Date of first publication: September 18, 2017

Keywords: Antiphospholipid syndrome and innate immunity

Session Information

Date: Monday, November 6, 2017

Title: Innate Immunity and Rheumatic Disease Poster II

Session Type: ACR Poster Session B

Session Time: 9:00AM-11:00AM

Background/Purpose:

The antiphospholipid syndrome (APS) is an autoimmune condition characterized by thrombosis, pregnancy complications and the presence of antiphospholipid antibodies. Basophils, which have long been associated with allergy and parasitic infections, are known to be activated and able to enhance the production of autoantibodies in autoimmune diseases, particularly in systemic lupus erythematosus (SLE). Herein we investigated the role of basophils in APS.

Methods:

Peripheral basophils of patients with primary APS, SLE-associated APS (SLE-APS), and healthy controls (HC) were analyzed using flow cytometry.

Results: Forty-three consecutive patients with APS, including 10 patients with SLE-APS, and 20 HC were recruited. None of the patients was treated with corticosteroids or immunosuppressants. Thirty-five (81.4%) patients had lupus anticoagulant, 33 (76.7%) had anti-cardiolipin antibodies, and 24 (55.8%) had anti-ß2 glycoprotein I. Twenty (46.5%) patients had obstetric APS, 20 (46.5%) patients experienced arterial thrombosis and 19 (44.2%) patients experienced venous thrombosis. Six patients (14%) had a catastrophic APS (CAPS). Basophil activation markers revealed a decreased proportion of CD193⁺ basophils in patients with APS vs HC (63.0% [27.3 – 85.0] vs 90.4% [74.1 – 97.1]; p < 0.05) and an increased mean fluorescence intensity (MFI) of CD69 within basophils in patients with APS vs HC (839 [436 – 1434] vs 318 [103 – 755]; p < 0.05). Basophils functional markers revealed a decreased proportion of CD62L⁺ basophils in APS vs HC (99.0% [95.6 – 100] vs 100% [99.6 – 100]; p < 0.05), and an increased proportion of HLA-DR⁺ basophils in APS vs HC (32.3% [16.8 – 77.2] vs 10.0% [3.4 – 21.7]; p < 0.05). There was no difference between patients with APS and patients with SLE-APS, or patients with CAPS. When compared to patients without obstetric APS, patients with obstetric APS (46.5%) had a decreased proportion of CD196⁺ basophils (25.5% [11.3 – 32.5] vs 31.9% [25.8 – 66.3]; p < 0.05), an increased proportion of CD63⁺basophils (34.8% [0.25 – 53.3] vs 7.4% [0 – 37.9]; p < 0.05), and an increased proportion of HLA-DR⁺ basophils (49.0% [23.4 – 79.6] vs 20.8% [8.7 – 70.8]; p < 0.05). Lastly, the proportion of CD154⁺ basophils was higher in patients who experienced miscarriages than those who did not (47.6% [38.2 – 56.1] vs 19.7%[5.1 – 35.8]; p < 0.01) and MFI of basophil CD62L was higher in patients who experienced fetal death in utero than those who did not (11823 [9862 – 15546] vs 7978 [4628 – 10160]; p < 0.05).

Conclusion:

Main phenotypic characteristics of basophils in APS are an increased expression of CD69 marker, a decreased expression of CD193 marker, and an increased expression of HLA-DR marker suggesting a role for basophils. Patients with obstetric APS differed from other APS patients by a skewed distribution of CD196⁺, CD63⁺and HLA-DR⁺ basophils.

Disclosure: B. Chaigne, None; V. Le Guern, None; T. A. Szwebel, None; R. Paule, None; C. Le Jeunne, None; N. Costedoat-Chalumeau, None; L. Mouthon, None.

To cite this abstract in AMA style:

Chaigne B, Le Guern V, Szwebel TA, Paule R, Le Jeunne C, Costedoat-Chalumeau N, Mouthon L. Phenotypic Characterization of Peripheral Basophil Perturbations in the Antiphospholipid Syndrome [abstract]. Arthritis Rheumatol. 2017; 69 (suppl 10). https://acrabstracts.org/abstract/phenotypic-characterization-of-peripheral-basophil-perturbations-in-the-antiphospholipid-syndrome/. Accessed .

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