Session Type: ACR Concurrent Abstract Session
Session Time: 2:30PM-4:00PM
Background/Purpose: IgA vasculitis (IgAV) represents a common systemic vasculitis in paediatric and adult population. Our current knowledge of disease pathogenesis is still very limited and information on miRNAs expression in IgAV is lacking. The aim of our study was to determine the expression of five miRNAs (miRNA-146a-5p, miRNA-148-3p, miRNA-155-5p, miRNA-223-3p and let-7b) in the affected skin of adult IgAV patients.
Methods: The study included 65 skin samples from consecutive, untreated IgAV patients (61.5% male, median age 67.6 years, range 29–91), and 20 samples of normal skin from healthy volunteers. Total RNA was isolated from tissue sections of formalin-fixed, paraffin-embedded samples. Expression of miRNAs was measured using qRT-PCR. To present relative miRNA expression, the ΔΔCT method was used. Skin miRNAs expression was correlated to clinical characteristics of adult IgAV patients.
Results: We found significantly higher levels of miRNA-155-5p, miRNA-223-3p and let-7b in the affected skin compared to controls (18.6-fold, 6.4-fold and 7.9-fold higher respectively). Contrary, the mi-RNA 148-3p expression was significantly lower (2.2-fold). The expression of the miRNA-146-5p was near normal levels. Patients with necrotic skin lesions had significantly higher miRNA-223 tissue expression than those with non-necrotic purpura (p=0.029). Gastrointestinal tract involvement inversely correlated with the expression of miRNA-155-5p (p=0.004) and/or miRNA-146a-5p (p=0.041) in affected skin. No significant association between renal involvement or its severity, and skin expression of the investigated miRNAs was found. Patients with elevated serum IgA had a borderline upregulated skin expression of miRNA 223-3p (p=0.053).
Conclusion: An altered expression of miRNA-148b-3p, miRNA-155-5p, miRNA-223-3p and let-7b was found in vasculitic skin lesions in IgAV. The study revealed potential associations between the selected miRNA expression patterns and specific disease manifestations. Aberrantly expressed miRNAs could represent a biomarker in adult IgAV.
To cite this abstract in AMA style:Hočevar A, Tomšič M, Pižem J, Bolha L, Sodin Semrl S, Glavač D. Microrna Expression in the Vasculitic Skin Lesions of Adult Patients with IgA Vasculitis [abstract]. Arthritis Rheumatol. 2018; 70 (suppl 10). https://acrabstracts.org/abstract/microrna-expression-in-the-vasculitic-skin-lesions-of-adult-patients-with-iga-vasculitis/. Accessed July 15, 2020.
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