ACR Meeting Abstracts

ACR Meeting Abstracts

Abstract Number: 287

Interleukin-35 in Idiopathic Inflammatory Myopathies

Herman F. Mann1, Hana Hulejova2,3, Josef Zámecník4, Jaromir Hacek5, Mária Filková6, Olga Kryštufková7, J Vencovsky8 and Ladislav Senolt9, 1Department of Experimental Rheumatology, Institute of Rheumatology, 1st Faculty of Medicine, Charles University in Prague, Prague 2, Czech Republic, 2Institute of Rheumatology and Department of Rheumatology, Prague, Czech Republic, 31st Faculty of Medicine, Charles University, Prague, Prague, Czech Republic, 4Department of Pathology and Molecular Medicine, 2nd Medical School and University Hospital Motol, Charles University, Prague, Czech Republic, 5Department of Pathology and Molecular Medicine, 2nd Medical School and University Hospital Motol, Charles University, Prague, Czech Republic, Prague, Czech Republic, 6Institute of Rheumatology and Department of Rheumatology, 1st Faculty of Medicine, Charles University in Prague, Prague, Czech Republic, 7Institute of Rheumatology, Department of Rheumatology of the First Faculty of Medicine, Charles University in Prague, Prague, Czech Republic, 8Institute of Rheumatology, Charles University, Prague, Czech Republic, 9Institute of Rheumatology, Prague, Czech Republic

Meeting: 2015 ACR/ARHP Annual Meeting

Date of first publication: September 29, 2015

Keywords:

Session Information

Date: Sunday, November 8, 2015

Title: Muscle Biology, Myositis and Myopathies Poster

Session Type: ACR Poster Session A

Session Time: 9:00AM-11:00AM

Background/Purpose: Interleukin-35 (IL-35) is a newly described heterodimeric cytokine that belongs to the IL-12 family and consists of p35 (IL-12a) and EBI3 (IL-27b) subunits. IL-35 exerts immunomodulatory activities in several human autoimmune inflammatory diseases. Our objective was to assess IL-35 expression in muscle tissue and serum levels of IL-35   in myositis patients compared to healthy controls and characterise its potential association with myositis disease activity. 

Methods: The expression of IL-35 was studied by primary rabbit anti-human EBI3 polyclonal antibody and mouse anti-human p35 monoclonal antibody in a series of 19 muscle biopsy samples of idiopathic inflammatory myopathies (9 dermatomyositis/DM, 10 polymyositis/PM) and 10 cases of non-inflammatory myopathies and 10 control muscles biopsies. Serum levels of IL-35 were determined in 28 DM, 26 PM and 17 cancer associated myositis patients as well as in 40 healthy controls. Disease activity was evaluated by the score of the Myositis Disease Activity Assessment Tool (MYOACT), including both extramuscular, muscular and the physician’s score of overall disease activity. 

Results: Both IL-35 subunits were found in immune cells of the inflammatory infiltrates in idiopathic inflammatory myopathies, but not in muscle cells. No immunoreactivity was observed in muscle tissue of healthy controls and in non-inflammatory myopathies. IL-35 serum levels were increased in all myositis patients compared to healthy controls (p< 0.001). There were no differences in IL-35 serum levels among myositis subgroups. In patients with PM, but not DM, serum IL-35 levels correlated with MYOACT score (r=0.548, p=0.023), lactate dehydrogenase (r=0.621, p=0.024), CRP (r=0.632, p=0.009) and physician’s score (r=0.514, p=0.042). 

Conclusion:

IL-35 subunits are overexpressed in inflamed muscle tissue and elevated circulating IL-35 levels are associated with several disease activity parameters in polymyositis patients. These data suggest potential role of IL-35 in the pathogenesis of inflammatory myopathies.

Acknowledgement: Supported by MHCR support for conceptual development of a research organization (023728) and BTCure (115142-2).

Disclosure: H. F. Mann, None; H. Hulejova, None; J. Zámecník, None; J. Hacek, None; M. Filková, None; O. Kryštufková, None; J. Vencovsky, MedImmune Ltd, 5; L. Senolt, None.

To cite this abstract in AMA style:

Mann HF, Hulejova H, Zámecník J, Hacek J, Filková M, Kryštufková O, Vencovsky J, Senolt L. Interleukin-35 in Idiopathic Inflammatory Myopathies [abstract]. Arthritis Rheumatol. 2015; 67 (suppl 10). https://acrabstracts.org/abstract/interleukin-35-in-idiopathic-inflammatory-myopathies/. Accessed .

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