Date: Sunday, October 21, 2018
Session Title: 3S079 ACR Abstract: Antiphospholipid Syndrome (851–856)
Session Type: ACR Concurrent Abstract Session
Session Time: 2:30PM-4:00PM
Background/Purpose: 1. To characterize the mRNAs and microRNAs transcriptomes of monocytes, key immune cells in the atherothrombotic pathology of Antiphospholipid Syndrome patients (APS). 2. To evaluate the role of antiphospholipid antibodies (aPL) in the regulation of these processes. 3. To investigate the short-term effects of in vivo ubiquinol (reduced coenzyme Q10 [Qred]) supplementation.
Methods: Monocytes from peripheral blood of 30 APS patients and 30 healthy donors were purified by negative immunomagnetic selection. Then, gene expression microarray (Agilent G4112F platform) and nCounter microRNA expression arrays (Nanostring) were performed. Functional categorization of altered genes and miRNAs was made using IPA software, and interaction networks were identified. Genes and miRNAs integrating the networks were validated in the whole APS cohort, as well as on a set of thrombotic non-autoimmune patients. Predicted miRNA-mRNA interactions were tested by microRNA over-expression experiments. The short-term effects of in vivo Qred supplementation on the monocyte transcriptomes profiles were further analyzed.
Results: Microarray identified 518 altered genes in APS monocytes. Relevant biofunctions on which these genes were involved included
Conclusion: 1. Gene and microRNA expression profiles allowed the identification of relevant genes and pathways altered in monocytes of APS patients, associated with the pathogenesis of the disease and modulated, at least partially, by
Funded by ISCIII, PI15/01333 and RIER RD16/0012/0015 co-funded with FEDER
To cite this abstract in AMA style:Perez-Sanchez C, Pérez Sánchez L, Patiño-Trives AM, Luque Tevar M, Scudeler L, Ibáñez-Costa A, Ruiz-Limon P, Jiménez-Gómez Y, Arias de la Rosa I, Abalos-Aguilera MC, Segui P, Barbarroja N, Villalba JM, Collantes Estevez E, Cuadrado MJ, Aguirre Zamorano MÁ, Lopez-Pedrera C. Integrated mRNA and microRNA Transcriptomes of Monocytes from Antiphospholipid Syndrome Patients Identifies Molecular Networks Related to Their Atherothrombotic Status. Modulatory Effects of In Vivo Ubiquinol Supplementation [abstract]. Arthritis Rheumatol. 2018; 70 (suppl 10). https://acrabstracts.org/abstract/integrated-mrna-and-microrna-transcriptomes-of-monocytes-from-antiphospholipid-syndrome-patients-identifies-molecular-networks-related-to-their-atherothrombotic-status-modulatory-effects-of-in-vivo-u/. Accessed January 21, 2020.
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