Session Information
Date: Tuesday, November 10, 2015
Title: Vasculitis Poster III
Session Type: ACR Poster Session C
Session Time: 9:00AM-11:00AM
Background/Purpose:
The immune mechanisms underlying Takayasu’s arteritis (TA) are not clear. Expanded Th17 T cell subset has been demonstrated in Giant Cell Arteritis. Gamma delta T cells (γδT), natural killer cells (NK) and natural killer -T cells (NKT) are also producers of IL-17 besides Th17 cells. Studies have demonstrated an increased number of circulating γδT cells in peripheral blood and in histopathology of arterial biopsies of patients with TA. We therefore enumerated Th17, γδT , NK and NKT cells and serum levels of IL-17 and IL23 in peripheral blood of patients with TA, and looked for its correlation with disease activity and erythrocyte sedimentation rate (ESR).
Methods: 30 patients with TA fulfilling ACR 1990 criteria and 20 healthy controls were included after seeking informed consent. NK cells (CD3-CD56+), NK-T cells (CD3+CD56+) and γδ-T cells (CD3+and γδTCR+) were surface stained in whole blood. For Th17 cells, 500 μl blood+500 μl cRPMI was cultured for 6 hours in presence of 50 ng/ml PMA, 1 μg/ml ionomycin for activation and golgi plug 0.2 μM monansin was added in last 4 hours of culture. Surface staining was done with anti-CD3, CD4, and intracellular staining for IL-17. Frequencies of all cell types were analysed by flow cytometry. Th17 cells could not be assessed in one patient due to technical difficulties.
IL-17 and IL-23 were measured in serum by ELISA according to manufacturer’s instructions. The relation of studied cell populations and serum IL-17 and IL-23 was analysed with disease activity (assessed by Kerr criteria, ITAS2010 and ITASA). Non-parametric tests were used for analysis (results presented as median with inter-quartile range). This study was approved by the institute ethics committee.
Results:
Mean age of patients was 33.47±11.78 years (25 females); mean symptom duration was 7.1±5.3 years. 13 were not on immunosuppressive drugs. 12 patients had ITAS ≥ 4. Compared with normal, the percentage of Th17 cells was significantly expanded in patients with TA. No differences in other cell populations were found. Serum IL-17 and IL-23 (pg/mL) in patients were significantly higher than controls (Table 1).
Subgroup analysis showed that the increased Th17 cells, serum IL-17 and IL-23 did not correlate with ESR, disease activity or medications.
Conclusion:
There is significant expansion of Th17 cells and elevation of serum IL-17 and IL-23 levels in patients with TA as compared to healthy controls. No relationship with disease activity could be demonstrated.
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Table 1 – Gamma delta T cells, NK cells, NKT cells , Th17 cells, serum IL-17 and serum IL-23 in patients and controls (median with interquartile range in brackets) |
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|
|
Patients (30) |
Healthy controls (20) |
P value |
|
Serum IL-17 (pg/mL) |
6.2 (4.6-8.5) |
3.9 (3.9-7.3) |
0.004 |
|
Serum IL-23 (pg/mL) |
15 (14.9-26.5) |
14.9 (14.9-14.9) |
<0.0001 |
|
Th17 cells (%) |
2.1 (1.5-3.2) (n=29) |
0.75 (0.32-1.2) |
<0.0001 |
|
Gamma delta T cells(%) |
5.5 (3.9-7.1) |
6 (2.7-9.9) |
0.72 |
|
NK cells(%) |
5.2 (3.6-8.7) |
6.9 (4.3-9.3) |
0.39 |
|
NKT cells(%) |
3.7 (1.9-6.5) |
2.4 (1.5-4.9) |
0.12 |
To cite this abstract in AMA style:
Misra R, Misra DP, Chaurasia S. Increased Circulating Th17 Cells, Serum IL-17 and Serum IL-23 in Takayasu’s Arteritis [abstract]. Arthritis Rheumatol. 2015; 67 (suppl 10). https://acrabstracts.org/abstract/increased-circulating-th17-cells-serum-il-17-and-serum-il-23-in-takayasus-arteritis/. Accessed .« Back to 2015 ACR/ARHP Annual Meeting
ACR Meeting Abstracts - https://acrabstracts.org/abstract/increased-circulating-th17-cells-serum-il-17-and-serum-il-23-in-takayasus-arteritis/