ACR Meeting Abstracts

ACR Meeting Abstracts

  • Meetings
    • ACR Convergence 2024
    • ACR Convergence 2023
    • 2023 ACR/ARP PRSYM
    • ACR Convergence 2022
    • ACR Convergence 2021
    • ACR Convergence 2020
    • 2020 ACR/ARP PRSYM
    • 2019 ACR/ARP Annual Meeting
    • 2018-2009 Meetings
    • Download Abstracts
  • Keyword Index
  • Advanced Search
  • Your Favorites
    • Favorites
    • Login
    • View and print all favorites
    • Clear all your favorites
  • ACR Meetings

Abstract Number: 1733

Immunomodulatory Effects of Dietary Non-Digestible Oligosaccharides in T Cell-Mediated Autoimmune Arthritis

Rebecca Rogier1, Tom Ederveen2, Anita Hartog3, Birgitte Walgreen4, Liduine van den Bersselaar1, Monique M. Helsen1, Paul Vos3, Johan Garssen3,5, Linette Willemsen5, Wim B. van den Berg1, Marije I. Koenders1 and Shahla Abdollahi-Roodsaz6, 1Experimental Rheumatology, Radboud university medical center, Nijmegen, Netherlands, 2Centre for Molecular Bioinformatics Nijmegen (CMBI), Radboud university medical center, Nijmegen, Netherlands, 3Danone Nutricia Research, Utrecht, Netherlands, 4Experimentel Rheumatology, Radboud university medical center, Nijmegen, Netherlands, 5Division of Pharmacology, Utrecht Institute for Pharmaceutical Sciences, Utrecht, Netherlands, 6Rheumatology, Radboud university medical center, Nijmegen, Netherlands

Meeting: 2014 ACR/ARHP Annual Meeting

Keywords: Autoimmunity, microbiome and rheumatoid arthritis (RA), T cells

  • Tweet
  • Click to email a link to a friend (Opens in new window) Email
  • Click to print (Opens in new window) Print
Session Information

Title: T cell Biology in Rheumatoid Arthritis and Other Arthritis

Session Type: Abstract Submissions (ACR)

Background/Purpose: Accumulating evidence indicates the relevance of intestinal microbiota in shaping the immune response and supports its contribution to the development of autoimmune diseases. Prebiotic non-digestible oligosaccharides are known to selectively support growth of commensal Bifidobacteria and Lactobacilli and adjust the microbiota composition. The aim of this study was to assess the efficacy of microbiota modulation using non-digestible oligosaccharides as a therapeutic approach for T cell-dependent autoimmune arthritis.

Methods: IL-1 receptor antagonist (IL-1Ra) deficient mice spontaneously developing an autoimmune T cell- and interleukin (IL)-17- dependent arthritis were used for this study. We previously showed that spontaneous arthritis in IL-1Ra-/- mice depends on the presence of commensal microbiota, since germ-free mice develop less severe disease. To examine the feasibility of microbiota modulation as a therapeutic approach during established disease, IL-1Ra-/- mice which had already developed arthritis under conventional microbial status were orally fed a prebiotic diet containing 2.5% or 5% short-chain galacto- and long-chain fructooligosaccharides (scGos:lcFos, 9:1). Disease progression was monitored and intestinal and systemic T cell differentiation was studied.

Results: Oral treatment of arthritic IL-1Ra-/- mice with scGoslcFos significantly suppressed the progression of arthritis. Furthermore, dual-energy X-ray absorptiometry scanning revealed that a prebiotic diet containing scGoslcFos significantly improved bone mineral density and tended to increase bone mineral content in arthritic IL-1Ra-/-mice.

Gene expression of T-bet and RORγt, the Th1 and Th17-related transcription factors, in lymph nodes draining the arthritic joints was significantly reduced in the group receiving the scGoslcFos diet. Flow cytometry analysis of the lymph nodes showed no effect on the percentages of Th1, Th17 and regulatory T cells (Tregs). However, the percentage of CD3+CD4+ IL-4 producing cells tended to be increased in the scGoslcFos treated group.

Interestingly, small intestine lamina propria of mice receiving scGoslcFos diet contained increased percentages of CD3+CD4+ FoxP3+regulatory T cells. In addition, intestinal gene expression of the Treg-related transcription factor FoxP3 as well as anti-inflammatory cytokine IL-10 were increased with scGoslcFos. Accordingly, small intestine lamina propria lymphocytes of mice receiving the 5% scGoslcFos diet produced significant higher levels of IL-10 upon ex vivo stimulation with PMA and ionomycin. Production of IL-4 and IFNγ also tended to be increased, while production of TNFα, IL-6 and IL-17 was not affected by the prebiotic diet.

Conclusion: Our data suggest that scGoslcFos suppresses arthritis progression, potentially through induction of anti-inflammatory cytokines such as IL-10 and IL-4. Suppression of disease progression using dietary intervention with prebiotic scGoslcFos may be applicable as a therapeutic approach to suppress autoimmune arthritis.


Disclosure:

R. Rogier,
None;

T. Ederveen,
None;

A. Hartog,
None;

B. Walgreen,
None;

L. van den Bersselaar,
None;

M. M. Helsen,
None;

P. Vos,
None;

J. Garssen,
None;

L. Willemsen,
None;

W. B. van den Berg,
None;

M. I. Koenders,
None;

S. Abdollahi-Roodsaz,
None.

  • Tweet
  • Click to email a link to a friend (Opens in new window) Email
  • Click to print (Opens in new window) Print

« Back to 2014 ACR/ARHP Annual Meeting

ACR Meeting Abstracts - https://acrabstracts.org/abstract/immunomodulatory-effects-of-dietary-non-digestible-oligosaccharides-in-t-cell-mediated-autoimmune-arthritis/

Advanced Search

Your Favorites

You can save and print a list of your favorite abstracts during your browser session by clicking the “Favorite” button at the bottom of any abstract. View your favorites »

All abstracts accepted to ACR Convergence are under media embargo once the ACR has notified presenters of their abstract’s acceptance. They may be presented at other meetings or published as manuscripts after this time but should not be discussed in non-scholarly venues or outlets. The following embargo policies are strictly enforced by the ACR.

Accepted abstracts are made available to the public online in advance of the meeting and are published in a special online supplement of our scientific journal, Arthritis & Rheumatology. Information contained in those abstracts may not be released until the abstracts appear online. In an exception to the media embargo, academic institutions, private organizations, and companies with products whose value may be influenced by information contained in an abstract may issue a press release to coincide with the availability of an ACR abstract on the ACR website. However, the ACR continues to require that information that goes beyond that contained in the abstract (e.g., discussion of the abstract done as part of editorial news coverage) is under media embargo until 10:00 AM ET on November 14, 2024. Journalists with access to embargoed information cannot release articles or editorial news coverage before this time. Editorial news coverage is considered original articles/videos developed by employed journalists to report facts, commentary, and subject matter expert quotes in a narrative form using a variety of sources (e.g., research, announcements, press releases, events, etc.).

Violation of this policy may result in the abstract being withdrawn from the meeting and other measures deemed appropriate. Authors are responsible for notifying colleagues, institutions, communications firms, and all other stakeholders related to the development or promotion of the abstract about this policy. If you have questions about the ACR abstract embargo policy, please contact ACR abstracts staff at [email protected].

Wiley

  • Online Journal
  • Privacy Policy
  • Permissions Policies
  • Cookie Preferences

© Copyright 2025 American College of Rheumatology