Background/Purpose:
Behcet’s disease (BD) is a chronic, multisystemic vasculitis, pathogenesis of BD remains enigmatic. Diagnosis of BD is sometimes difficult, and until now, no specific serological markers have been established. Identification of antigens incorporated into CICs provides information that may be helpful in developing diagnostic and treatment strategies for autoimmune diseases, and such information might be more relevant than information on free antigens.The purpose of this study is to seek candidate autoantigens in CIC in BD, analyze autoantiges through Orbitrap mass spectrometry, and detect antibody levels of candidate autoantigens through ELISA in different autoimmune diseases.
Methods:
A novel proteomic strategy (immune complexome analysis) was developed, in which circulating immune complexes (CICs) were separated from pooling serum sample of 10 BD patients and 10 healthy controls, digested directly with trypsin, and then subjected to Orbitrap mass spectrometry for identifying and profiling antigens in CICs. Anti-tubulin- a -1C antibody level were detected by Enzyme-linked immunosorbent assay (ELISA) in sera of 44 BD, 51 SLE, 51 SSc, 40 RA, 51 SS, 22 RAU, 13 TA, 25 AASV and 59 healthy volunteers.
Results:
A totle of 22 antigens incorporated into CICs were found in CICs of BD patients, but not in RA and HC, including TAOK3, BAG3 and Tubulin-a-1C et al. The auto-antibody to one of the identified proteins, Tubulin-a-1C, was investigated by ELISA using a recombinant protein. The auto-antibody to Tubulin-a-1C were detected positive in 26 (59.0%) of the 44 patients with BD, 14 (27.5%) of the 51patients with SLE, 14 (27.5%) of the 51patients with SSc, 3 (7.5%) of the 40 patients with RA, 3(23.1%) of 13 TA, 1(4%) of 25 AASV and 2（3.39%）of 59 HC. The sensitivity and specificity of Tubulin-a-1C antibody in the diagnosis of BD were 59.0% and 82.7% respectively. Further analysis demonstrated that Tubulin-a-1C antobody was correlated with complications of deep venous thrombosis and erythema nodosum of BD(p<0.05), and meanwhile,levels of anti- Tubulin-a-1C antibody were correlated with levels of ESR , CRP and BVAS(p<0.05).
Conclusion: Anti- Tubulin-a-1C antibody may be helpful in diagnosis and severity evaluation of BD, CIC relevant antigens may benefit understanding of the pathogenesis of venous thrombosis and erythema nodosum of BD.
Disclosure:
Y. CHENG Jr.,
None;
X. Sun,
None;
Y. Chen Jr.,
None;
C. Huang,
None;
H. Deng,
None;
Z. G. Li,
None.
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ACR Meeting Abstracts - https://acrabstracts.org/abstract/identification-of-potential-serum-biomarkers-for-behcet-disease-by-high-resolution-quantitative-proteomic-analysis/