Session Information
Session Type: ACR Poster Session B
Session Time: 9:00AM-11:00AM
Background/Purpose: Pertussis cases have increased worldwide and knowledge on immune response and cytokine profile after adult Tdap vaccine is scarce. This study evaluated the humoral and cellular response through antibodies and cytokine profile after Tdap in juvenile systemic lupus erythematosus (jSLE) and controls.
Methods: After written informed consent, jSLE (n=20) and healthy adolescents (n=8) who received three whole-cell DTP vaccine (DTwP) plus two booster doses and had lymphocytes count > 500 received a Tdap dose. Blood samples were collected immediately before and 28 days after the vaccine. In vitro culture was performed with whole blood stimulated with tetanus toxoid, Bordetella pertussis or medium. Supernatants were collected after 7 days, kept frozen and subsequently assayed for cytokines secretion by xMAP-Luminex platform. Tetanus, diphtheria and pertussis antibodies were tested by ELISA.
Results: jSLE patients group median age was 14y (9-18y) and control group, 15.2y (9-15.6y). Both control group and jSLE presented an increase in response to tetanus (p<0.01), diphtheria (p<0.001) and pertussis (p=0.01) antibodies after Tdap booster dose on day 28. Pertussis cellular immune response on day 28 was stronger in control group, with significantly higher cytokine levels for IL-2, IL-6, IL12p70, IFN-gamma, and TNF-alpha; by contrast, IL-4 concentration was higher in jSLE. Tetanus cellular immune response on day 28 was also stronger in control group, with significantly higher cytokine concentrations for IL-2, IL-4, and IFN-gamma; however, IL-12p70 and TNF-alpha were higher in jSLE group. The cytokine levels and p-values are shown in table 1.
| Table 1 – Cytokine levels for pertussis and tetanus cellular immune response | |||||
|
Pertussis |
|||||
|
|
jSLE D0 |
jSLE D 28 |
Control D0 |
Control D28 |
D28 p-value |
|
IL-2 |
2.26 |
0.28 |
1.29 |
1.16 |
0.0201 |
|
IL-4 |
2,276.37 |
1,293.05 |
0.83 |
1.1 |
0.0019 |
|
IL-6 |
281.92 |
265.69 |
1,928.02 |
2,913.48 |
0.0019 |
|
IL-10 |
61.16 |
43.92 |
33.88 |
53.24 |
0.5186 |
|
IL-12p70 |
13.36 |
2.34 |
1.69 |
9.33 |
0.014 |
|
IFN-gamma |
18.99 |
46.16 |
186.21 |
536.04 |
0.0098 |
|
TNF-alpha |
21.63 |
14.81 |
158.72 |
228.49 |
0.0019 |
|
Tetanus |
|||||
|
|
jSLE D0 |
jSLE D 28 |
Control D0 |
Control D28 |
D28 p-value |
|
IL-2 |
3.19 |
1.59 |
4.33 |
10.22 |
0.0528 |
|
IL-4 |
2,451.86 |
1,323 |
2,291.08 |
3,784 |
0.0142 |
|
IL-6 |
361.79 |
219 |
172.14 |
359 |
0.1556 |
|
IL-12p70 |
21.73 |
11.61 |
2.16 |
2.25 |
0.0067 |
|
IL-10 |
49.24 |
41.46 |
24.95 |
57.09 |
0.2453 |
|
IFN-gamma |
29.43 |
54.31 |
219.05 |
2,152.06 |
0.0067 |
|
TNF-alpha |
18.02 |
12.47 |
2.27 |
1.3 |
0.0142 |
Conclusion: In this preliminary study, children and adolescents with jSLE and healthy controls showed adequate humoral immune response to tetanus, diphtheria and pertussis after Tdap. However, jSLE adolescents showed a Th2 pertussis immune response profile, what might not be adequate to control B. pertussis infection.
To cite this abstract in AMA style:
Peracchi O, Nicacio A, Spina F, Yamada J, Alvarenga B, Pinto MI, Terreri MT. Humoral Immune Response and Cytokine Profile after a Booster Dose with Tdap Vaccine in Juvenile Systemic Lupus Erythematosus and Controls [abstract]. Arthritis Rheumatol. 2016; 68 (suppl 10). https://acrabstracts.org/abstract/humoral-immune-response-and-cytokine-profile-after-a-booster-dose-with-tdap-vaccine-in-juvenile-systemic-lupus-erythematosus-and-controls/. Accessed .« Back to 2016 ACR/ARHP Annual Meeting
ACR Meeting Abstracts - https://acrabstracts.org/abstract/humoral-immune-response-and-cytokine-profile-after-a-booster-dose-with-tdap-vaccine-in-juvenile-systemic-lupus-erythematosus-and-controls/