Session Information
Session Type: Abstract Submissions (ACR)
Background/Purpose: Sjogren’s syndrome (SS) is an autoimmune disease with abnormally infiltrating lymphocytes in exocrine glands expressing Th1 cytokines and increased incidence of lymphoma. Histone deacetylases (HDACs) regulate the state of acetylation of histones and many other intracellular proteins, and their role in the pathogenesis of malignancies has been established. HDAC1 and 2 may also affect the balance of Th1 and Th2 immune response. The aim of this study is to investigate whether HDAC1 and 2 are abnormally expressed in the salivary glands of SS.
Methods: Labial gland biopsies were performed in 55 patients, among them 25 were diagnosed as SS. The expression of HDAC1 and 2 were investigated by immunohistochemistry and immunofluorescence (IF). The HDACs expression level was semi-quatatvely analyzed by counting the number of positively stained cells in randomly chosen microscopic fields. The difference of HDAC expression level in different locus was analyzed by t test.
Results: HDAC1 was highly expressed in the infiltrating lymphocytic foci in the labial glands of patients with SS. The proportion of HDAC1+ cells in lymphocytic foci was higher than that in interstitium (0.66±0.24 vs 0.26±0.08, p<0.01). Concerning the expression of HDAC1 in lymphocytes outside the foci, the number of HDAC1+ cells in the interstitium of labial glands was higher in SS patients than that in non-SS patients (21±11/hpf vs 11±6/hpf, p<0.05). IF showed that HDAC1 was mainly expressed in CD4+ T cells. HDAC2 was not detected in the labial glands comparing positive control staining of tonsil tissue. The expression level of HDAC1 was not associated with the IgG level and presence of autoantibodies in patients with SS.
Conclusion: The expression of HDAC1 is elevated in the lymphocytic foci in SS and may be involved in its pathogenesis. Thus, inhibiting HDAC1 specifically is a potential therapeutic strategy of SS that is worthy of further investigation.
Disclosure:
J. Guo,
None;
W. Zhou,
None.
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ACR Meeting Abstracts - https://acrabstracts.org/abstract/histone-deacetylase-1-but-not-2-is-highly-expressed-in-the-lymphocyte-foci-in-the-labial-glands-of-patients-with-sjogrens-syndrome/