Session Type: ACR Poster Session B
Session Time: 9:00AM-11:00AM
Background/Purpose: Obesity is associated with many chronic diseases. However, evidence regarding obesity’s impact on rheumatoid arthritis (RA) initiation and development remains controversial. Recent studies indicate that obesity could reduce the risk of men developing RA. Here, we examined the role of high fat diet (HFD)-induced obesity in antigen-induced arthritis in adult male mice.
Methods: Antigen-induced arthritis (AIA) was induced in normal diet (ND) or 8-week exposure HFD mice by immunizing with mBSA in complete Freund’s adjuvant. Arthritis severity was assessed by knee swelling measurement and histological evaluation. Serum anti-mBSA antibody and cytokines were detected by ELISA or Multiplex bead assay at various time points. T cell subpopulations and B regulatory cell in spleen and lymph node were evaluated in vivo, and their function was also examined by measuring cytokines production after phorbol ester (PMA) and Ionomycin stimulations in vitro.
Results: When compared to ND-mice, HFD-mice displayed less knee swelling and delayed peak arthritis. Consistent with these observations, histological assessment showed reduced infiltration of inflammatory cell and bone erosion in obese mice joints. Despite no difference in TNF-a, IL-21, IL-22 and TGF-b1 level in sera, IL-21/23p40 and anti-mBSA IgG production was significantly lower in HFD mice. Interestingly, the anti-inflammatory cytokine IL-10 level was increased in sera from HFD mice. Surprisingly, Th1, Th2 and Th17 cell population did not differ in both spleen and lymph node. Consistently, levels of IFN-g, IL-4 and IL-17A were comparable in the splenocyte supernatant from ND and HFD mice. Interestingly, a significantly increased Treg population was detected in the spleen of HFD mice but not in the lymph node. CD19+IL-10+ (B10) cells in the spleen of HFD mice were also enhanced, accompanied by higher IL-10 level in the splenocyte supernatant in vitro.
Conclusion: In summary, these data suggest HFD-induced obesity alleviates AIA-induced arthritis in male mice. This might due to the enhanced Treg and B10 cell population in HFD mice, which leads to increased IL-10 in sera of obese mice. This study provides a novel mechanistic link of obesity and RA.
To cite this abstract in AMA style:Luo Y, Engdahl C, Zaho Y, Liu Y, Schett G, Bozec A. High Fat Diet Alleviates Antigen-Induced Arthritis Severity in Male Mice By Enhancing Both Treg and B10 Cells [abstract]. Arthritis Rheumatol. 2016; 68 (suppl 10). https://acrabstracts.org/abstract/high-fat-diet-alleviates-antigen-induced-arthritis-severity-in-male-mice-by-enhancing-both-treg-and-b10-cells/. Accessed August 8, 2020.
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ACR Meeting Abstracts - https://acrabstracts.org/abstract/high-fat-diet-alleviates-antigen-induced-arthritis-severity-in-male-mice-by-enhancing-both-treg-and-b10-cells/