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Abstract Number: 913

High Erythrocyte Levels of the n-6 Polyunsaturated Fatty Acid Linoleic Acid Are Associated with Lower Risk of Subsequent Rheumatoid Arthritis in a Southern European Nested Case-Control Study

Paola de Pablo1, Dora Romaguera2,3, Helena Fisk4, Philip Calder4, Anne-Marie Quirke5, Alison Cartwright5, Salvatore Panico6, Amalia Mattiello6, Diana Gavrila7, Carmen Navarro7, Carlotta Sacerdote8, Paolo Vineis2,9, Rosario Tumino10, William Ollier11, Dominique Michaud2,12, Elio Riboli2, Patrick Venables5 and Benjamin Fisher13, 1University of Birmingham, Birmingham, United Kingdom, 2Imperial College London, London, United Kingdom, 3CIBER-OBN (Fisiopatología de la Obesidad y Nutrición), University Hospital Son Espases, Palma, Spain, 4University of Southampton, Southampton, United Kingdom, 5Kennedy Institute of Rheumatology, University of Oxford, Oxford, United Kingdom, 6Federico II University of Naples, Naples, Italy, 7Murcia Regional Health Council, Murcia, Spain, 8Città della Salute e della Scienza University-Hospital, Turin, Italy, 9Human Genetics Foundation, Turin, Italy, 10"Civic - M.P.Arezzo" Hospital, Ragusa, Italy, 11Division of Population Health, Health Services Research and Primary Care, University of Manchester, Manchester Academic Health Science Centre, Manchester, United Kingdom, 12Tufts University Medical School, Boston, MA, 13Rheumatology Research Group, University of Birmingham, Birmingham, United Kingdom

Meeting: 2017 ACR/ARHP Annual Meeting

Date of first publication: September 18, 2017

Keywords: dietary supplements, Epidemiologic methods, Lipids, rheumatoid arthritis (RA) and risk

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Session Information

Date: Sunday, November 5, 2017

Session Title: Epidemiology and Public Health II: Non-Genetic Risk Factors for Incident Disease

Session Type: ACR Concurrent Abstract Session

Session Time: 4:30PM-6:00PM

Background/Purpose:

Long-chain n-3 (also known as omega-3) polyunsaturated fatty acids (PUFA), especially eicosapentaenoic acid (EPA; 20:5n-3) and docosahexaenoic acid (DHA; 22:6n-3), have long-been considered to have anti-inflammatory and immunomodulatory actions. Both n-3 and n-6 series PUFA (determined by the number of carbon atoms between the methyl end of the fatty acyl chain and the first double-bond) cannot be synthesized de novo by animals. Findings relating to the dietary intake of n-3 PUFA and risk of rheumatoid arthritis (RA) are mixed. We compared erythrocyte membrane PUFA, as an accurate biomarker of PUFA status, between pre-RA individuals and matched controls from a population-based sample.

Methods:

EPIC is a multicentre, pan-European prospective cohort study of apparently healthy populations. We undertook a nested case-control study, by identifying RA cases with onset after enrolment (pre-RA) in four EPIC cohorts: Naples, Turin and Ragusa in Italy, and Murcia in Spain. Identification and case validation has been previously described [1]. Confirmed pre-RA cases were matched with controls by age, sex, centre, and date, time and fasting status at blood collection. Total erythrocyte lipids were extracted and dissolved in toluene. Fatty acid methyl esters were synthesied and resolved in a BPX-70 fused silica capillary column using an Agilent 6890 gas chromatograph equipped with flame ionisation detection. We also measured the following serum cytokines: TNFα, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12p70, IL-13 and IFNγ (Meso Scale Diagnostics). Conditional logistic regression (CLR) analysis of data was adjusted for potential confounders including body mass index (BMI), waist circumference, education level, physical activity, smoking status, and alcohol intake. Negative binomial regression was used to test the relationship between PUFA and serum cytokine level, stratified by incident RA, and adjusting for age, sex, country of origin, BMI, and smoking status.

Results:

The study analysed pre-symptomatic samples from 354 individuals of which 96 individuals went on to subsequently develop RA. In this analysis, time to diagnosis (defined as time between date of blood sample and date of diagnosis), was 6.71 years (SD 3.43).

No association was observed for any individual n-3 PUFA, or with total n-3 PUFA or total long chain n-3 PUFA, or with total n-3/n-6 ratio, and risk of RA. However a significant inverse association was observed with the n-6 PUFA linoleic acid (LA) level and pre-RA in the fully adjusted model (highest tertile : OR 0.29; 95% CI 0.12 to 0.75; p for trend 0.01).

Among the controls, LA was positively associated with serum levels of TNFα and IL-6 and negatively with IL-4, IL-5, IL-10, IL-12, IL-13 and IFNγ in fully adjusted models. In the pre-RA population, LA was positively associated with TNFα and IL-1, and negatively with IL-6, IL-5, IL-12 and IL-13 levels.

Conclusion:

In this nested case-control study within southern European prospective cohorts, high erythrocyte levels of the n-6 PUFA LA are associated with lower levels of T cell related cytokines and with lower risk of subsequent RA.

[1] Fisher BA, et al. BMC Musculoskelet Disord. 2015;16:331.



Disclosure: P. de Pablo, None; D. Romaguera, None; H. Fisk, None; P. Calder, None; A. M. Quirke, None; A. Cartwright, None; S. Panico, None; A. Mattiello, None; D. Gavrila, None; C. Navarro, None; C. Sacerdote, None; P. Vineis, None; R. Tumino, None; W. Ollier, None; D. Michaud, None; E. Riboli, None; P. Venables, None; B. Fisher, None.

To cite this abstract in AMA style:

de Pablo P, Romaguera D, Fisk H, Calder P, Quirke AM, Cartwright A, Panico S, Mattiello A, Gavrila D, Navarro C, Sacerdote C, Vineis P, Tumino R, Ollier W, Michaud D, Riboli E, Venables P, Fisher B. High Erythrocyte Levels of the n-6 Polyunsaturated Fatty Acid Linoleic Acid Are Associated with Lower Risk of Subsequent Rheumatoid Arthritis in a Southern European Nested Case-Control Study [abstract]. Arthritis Rheumatol. 2017; 69 (suppl 10). https://acrabstracts.org/abstract/high-erythrocyte-levels-of-the-n-6-polyunsaturated-fatty-acid-linoleic-acid-are-associated-with-lower-risk-of-subsequent-rheumatoid-arthritis-in-a-southern-european-nested-case-control-study/. Accessed January 21, 2021.
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