Session Title: Rheumatoid Arthritis: Human Etiology and Pathogenesis II
Session Type: Abstract Submissions (ACR)
Background/Purpose: In vitro and in vivo studies suggest that leptin and adiponectin are involved in the development of inflammation in RA patients. Previous findings by our group have shown that levels of these adipokines are significantly higher in RA in comparison with healthy patients, and correlate with variations in DAS28 after two years of follow-up. We have observed that high levels of adiponectin at baseline predict a positive response to DMARD treatment after 6-12 months. However, the biological mechanism of leptin and adiponectin on T cell physiology is still poorly understood. Objetive: To compare the in vitro proliferative and activation effects of leptin and adiponectin on mononuclear (MN) and CD4+ T cells from healthy donors and RA patients. Methods: All RA patients included in the present study fulfilled the ACR 2010 criteria and were followed at the rheumatology clinic. Healthy donors samples were obtained from a local blood bank. Mononuclear cells were obtained by Ficoll Paque the same day the blood was obtained. CD4+T cells were magnetically purified (Miltengy). In vitro assays were done employing recombinant human leptin and adiponectin (Peprotech). Proliferation was evaluated by MTT technique. Activation was evaluated by flow cytometry (CD25, CD69) and by ELISA (IL-1b, IL-2, IL-6, TNF-a). Th17 differentiation was evaluated by ELISA/cytometry (IL-17A) and western blot (RORyT). Descriptive statistics were employed to evaluate differences between groups and Spearman correlation was used to associate them with clinical parameters. A p-value <0.05 was considered as statistically significant. Results: MN and CD4+T cells from RA patients showed a significantly higher proliferative effect when exposed to leptin and adiponectin in comparison with healthy donors. According with these, leptin and adiponectin treatment were able to rescue MN cells from apoptosis induced by etoposide by aIL-2
, with respect to their effects on proliferation, activation and Th17 differentiation. Additionally, clinical activity correlated with the proliferative effect and IL-2 secretion induced by treatment with leptin.
C. Bustos-Rivera Bahena,
J. L. Montiel-Hernandez,
« Back to 2013 ACR/ARHP Annual Meeting
ACR Meeting Abstracts - https://acrabstracts.org/abstract/effects-of-leptin-and-adiponectin-on-proliferation-and-activation-were-are-significantly-increased-in-cd4-t-cells-from-rheumatoid-arthritis-patients/