Date: Sunday, November 8, 2015
Session Type: ACR Poster Session A
Session Time: 9:00AM-11:00AM
Considering the extent, predicted growth rates, and growing economic impact on health care, osteoporosis is a significant problem. In addition to hormones and nutrients, the immune system is suspected to play key roles in osteoporosis, primarily through the involvement of pro-inflammatory cytokines. Cytokines play important roles in the regulation of normal bone remodeling, as well as in bone resorption and formation during pathologic bone remodeling. The objective of this study was to measure levels of osteoclastogenesis stimulator cytokines TNF-a, IL-6, IL-12, IL-17 and osteoclastogenesis inhibitor cytokines IFN-g, IL-4, IL-10, IL-13 produced by peripheral blood mononuclear cells (PBMC) from postmenopausal osteoporotic women and normal healthy controls.
The study population included 36 postmenopausal women that were grouped based on their bone mineral density (BMD) into normal (n=12), osteopenia (n=16) and osteoporosis (n=8). PBMC from these subjects were stimulated with the mitogen phytohemagglutinin and culture supernatants collected after incubation were used to measure the levels of 8 cytokines using the Multiplex system (Millipore) read on the Magpex ELISA platform.
PBMC from osteopenic women produced significantly higher levels of IL-6 and IL-17A as compared to women with normal BMD (p=0.037, 0.026), while osteoporotic women produced significantly lower levels of IL-4 and IL-13 as compared to women with normal BMD (p=0.013, 0.048) as well as women with osteopenia (p=0.049, 0.006), which is suggestive of a decreased anti-inflammatory or increased pro-inflammatory cytokine bias. Furthermore, osteoporotic women produced higher levels of the pro-inflammatory cytokine TNF-a (p=0.021), and lower levels of the anti-inflammatory cytokine IL-10 (p=0.033) compared to osteopenic women. Ratios of osteoclastogenesis stimulator cytokines to osteoclastogenesis inhibitor cytokines suggest a dominance of osteoclastogenesis stimulator cytokines in postmenopausal women with lower BMD.
Conclusion: These data are suggestive of a bias towards increased osteoclastogenesis stimulator cytokines and pro-inflammatory cytokines in women with osteoporosis.
To cite this abstract in AMA style:Al-Jarallah K, Azizieh F, Shehab D, Reghupathy R, Gupta R. Cytokine Production Patterns in Osteoporosis Suggest a Pro-Inflammatory Bias [abstract]. Arthritis Rheumatol. 2015; 67 (suppl 10). https://acrabstracts.org/abstract/cytokine-production-patterns-in-osteoporosis-suggest-a-pro-inflammatory-bias/. Accessed August 24, 2019.
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