Session Title: Sjögren's Syndrome - Poster I: Translational Science
Session Type: ACR Poster Session A
Session Time: 9:00AM-11:00AM
Background/Purpose: Background Evidence suggests that levels of different cytokines in primary Sjögren’s syndrome (pSS) are associated with cell infiltration degree within salivary and lacrimal glands and severity of the disease. Chemokines appear to contribute to the pathogenesis of pSS. Objectives To evaluate the association between serum and salivary Th1/Th2/Th17 cytokine and chemokine leves and disease activity in patients with pSS.
Methods: Case-control study including 18 patients with pSS and 13 healthy controls. Disease activity was assessed by EULAR Sjögren’s Syndrome Disease Activity Index (ESSDAI); serum and salivary IFN-γ, TNF, IL-2, IL-4, IL- 6, IL-10, IL-17, IP10, MCP-1, MIG, RANTES and IL-8 levels were measured by bead-based assays following the sandwich immunoassay principle (BD Cytometric Bead Array; BD Biosciences). Differences between groups were analyzed using the Mann-Whitney U test. Correlation was estimated by Spearman ́s correlation coefficient.
Results: IL-17, IL-2, IL-4, IFNγ, MIG and RANTES salivary levels were statistically different between controls and patients with pSS (p <0.005). A significant correlation (p <0.005) was shown in patients between serum IL-6 and serum IL- 12 (r=0.004), IL-10 (r=0.006); serum IL-12 and serum RANTES (r=0.008); serum IP-10 and serum MIG (r=0.000), RANTES (r=0.003); serum MIG and serum RANTES (r=0.014); salivary IL-17 and salivary IL-2 (r=0.004), IL-4 (r=0.001), INFγ (r=0.000), TNF (r=0.002), IL-10 (r=0.000); salivary IL-6 and salivary IL-2 (r=0.000), IL-4 (r=0.000), TNF (r=0.000), IL- 10 (r=0.000), IL-8 (r=0.0003), MCP-1 (r=0.007); salivary IL2 and salivary IL4 (r=0.000), INFγ (r=0.006), TNF (r=0.001), IL10 (r=0.000); salivary IL4 and sallivary TNF (r=0.001), IL10 (r=0.000), MCP-1 (r=0.009); salivary TNF and salivary INFγ (r=0.002), IL-10 (r=0.000), IP-10 (r=0.006); salivary MCP-1 and salivary TNF (r=0.000), IL-10 (r=0.001), IL-8 (r=0.003).
Conclusion: Increased salivary Th1/Th2/Th17 cytokine and chemokine levels were found in pSS. No correlation was found between serum or salivary cytokine and chemokine levels and pSS disease activity.
To cite this abstract in AMA style:Tzec-Pérez A, Barbosa-Cobos RE, Lugo-Zamudio GE, Becerril-Mendoza LT. Cytokine and Chemokine Levels in Serum and Saliva As Disease Activity Biomarkers in Patients with Primary Sjögren’s Syndrome [abstract]. Arthritis Rheumatol. 2016; 68 (suppl 10). https://acrabstracts.org/abstract/cytokine-and-chemokine-levels-in-serum-and-saliva-as-disease-activity-biomarkers-in-patients-with-primary-sjogrens-syndrome/. Accessed October 20, 2020.
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