Comparison between IgG and IgM Type Anti-Alpha-Enolase Antibody in Patients with Behcet's Disease According to the Disease Severity

Shin Eui Kang¹, Sang Jin Lee^1,2, Hyun Jung Yoo^1,3, Jeong Yeon Kim¹, Ji Soo Park¹, Sehui Shon⁴, Eun Young Lee³, Eun Bong Lee³ and Yeong Wook Song^1,3, ¹Department of Molecular Medicine and Biopharmaceutical Sciences, Graduate School of Convergence Science and Technology, and College of Medicine, Seoul National University, Seoul, Korea, Republic of (South), ²Division of Rheumatology, Department of Internal Medicine, Kyungpook National University School of Medicine, Daegu, Korea, Republic of (South), ³Division of Rheumatology, Department of Internal Medicine, Seoul National University Hospital, Seoul, Korea, Republic of (South), ⁴Department of Molecular Medicine and Biopharmaceutical Sciences, Department of Molecular Medicine and Biopharmaceutical Sciences, Graduate School of Convergence Science and Technology, and College of Medicine, Seoul National University, Seoul, Korea, Republic of (South)

Meeting: ACR/ARHP Annual Meeting 2017

Date of first publication: September 18, 2017

Keywords: Behcet's syndrome and autoantibodies

Session Information

Date: Tuesday, November 7, 2017

Title: Vasculitis Poster III: Other Vasculitis Syndromes

Session Type: ACR Poster Session C

Session Time: 9:00AM-11:00AM

Background/Purpose:

Behcet’s disease (BD) is a chronic inflammatory disease of unknown etiology, characterized by recurrent oral and genital ulcers, skin lesions, uveitis, and arthritis. It is regarded as vasculitis and anti-endothelial cell antibodies are found in BD. One of the endothelial cell antibodies was reported to recognize alpha-enolase and serum IgM anti-alpha-enolase antibody (AEA) was associated with involvement of the vascular system in BD in previous study. This study was aimed to investigate expression of alpha-enolase in the surface of peripheral blood cells and serum IgM or IgG AEA, and their association with clinical manifestations or disease activity of BD.

Methods:

Cell surface alpha-enolase expression was examined from several cell types of peripheral blood, including lymphocytes, monocytes, and granulocytes using flow cytometry in patients with BD and healthy controls (HCs). Serum AEA levels were measured by enzyme-linked immunosorbent assay (ELISA) in 110 BD patients and age/sex matched 110 HCs. Association of alpha-enolase or AEA with clinical manifestation was analyzed.

Results:

The frequency of surface alpha-enolase-expressing cells was increased in BD in lymphocytes and monocytes. Serum IgG AEA levels were increased in BD patients (median [IQR], 0.360 [0.268-0.482], p < 0.0001), particularly in active patients (BDCAF ≥ 2) (0.375 [0.274-0.537], p < 0.0001) compared to HCs (0.274 [0.231-0.357]). The levels of IgG AEA were correlated with the number of oral ulcer, ESR, and CRP. There was no difference of IgM AEA in sera from BD compared to HCs (0.538 [0.351-0.763] vs. 0.504 [0.344-0.823], p = 0.764) and no association of AEA levels and clinical manifestations in patients with BD.

Conclusion:

Serum IgG AEA was increased in BD patients and correlated with oral ulcer, ESR and CRP.

Disclosure: S. E. Kang, None; S. J. Lee, None; H. J. Yoo, None; J. Y. Kim, None; J. S. Park, None; S. Shon, None; E. Y. Lee, None; E. B. Lee, None; Y. W. Song, None.

To cite this abstract in AMA style:

Kang SE, Lee SJ, Yoo HJ, Kim JY, Park JS, Shon S, Lee EY, Lee EB, Song YW. Comparison between IgG and IgM Type Anti-Alpha-Enolase Antibody in Patients with Behcet’s Disease According to the Disease Severity [abstract]. Arthritis Rheumatol. 2017; 69 (suppl 10). https://acrabstracts.org/abstract/comparison-between-igg-and-igm-type-anti-alpha-enolase-antibody-in-patients-with-behcets-disease-according-to-the-disease-severity/. Accessed .

ACR Meeting Abstracts - https://acrabstracts.org/abstract/comparison-between-igg-and-igm-type-anti-alpha-enolase-antibody-in-patients-with-behcets-disease-according-to-the-disease-severity/