ACR Meeting Abstracts

ACR Meeting Abstracts

  • Home
  • Meetings Archive
    • ACR Convergence 2022
    • ACR Convergence 2021
    • ACR Convergence 2020
    • 2020 ACR/ARP PRSYM
    • 2019 ACR/ARP Annual Meeting
    • 2018 ACR/ARHP Annual Meeting
    • 2017-2009 Meetings
    • Download Abstracts
  • Keyword Index
  • Advanced Search
  • Your Favorites
    • Favorites
    • Login
    • View and print all favorites
    • Clear all your favorites
  • Meeting Resource Center

Abstract Number: 1934

Chondrocytes Derived from Mesenchymal Stem Cells Differentiated in the Presence of Plasma-Derived Extracellular Vesicles from Osteoarthritic Patients Express Disease-Related Genes

Bartijn C.H. Pieters1, Onno J. Arntz1, Arjen B. Blom2, Peter M. van der Kraan2 and Fons A.J. van de Loo2, 1Experimental Rheumatology, Radboudumc, Nijmegen, Netherlands, 2Experimental Rheumatology, Radboud university medical center, Nijmegen, Netherlands

Meeting: 2017 ACR/ARHP Annual Meeting

Date of first publication: September 18, 2017

Keywords: chondrocytes, osteoarthritis and stem cells

  • Tweet
  • Email
  • Print
Session Information

Date: Tuesday, November 7, 2017

Session Title: Biology and Pathology of Bone and Joint Poster II

Session Type: ACR Poster Session C

Session Time: 9:00AM-11:00AM

Background/Purpose: Osteoarthritis (OA) is an age-related musculoskeletal disease and the most common form of arthritis characterized by low grade synovial inflammation and articular cartilage degeneration. Many different cell types have been implicated in the pathogenesis of OA and interplay between these cells contribute to the dynamic events associated with disease. Recently, extracellular vesicles (EVs) have emerged as mediators of intercellular communication. Being carriers of proteins, genetic information and metalloproteinases, their involvement in many diseases has now been established. There is however very little known about the pathophysiological role of EVs in musculoskeletal diseases. In this study, we investigated the effect of plasma EVs from OA patients during chondrogenic differentiation of mesenchymal stem cells (MSCs).

Methods: Plasma-derived extracellular vesicles (pEVs) were isolated from plasma of OA patients and age-matched healthy controls using size-exclusion chromatography. EV containing fractions were characterized according to ISEV guidelines [1]. Pelleted MSCs were stimulated with TGF-β and BMP-2 to induce chondrogenic differentiation, either in the presence of pEVs isolated from OA patients or healthy controls. After 8 days, RNA was isolated and RT-qPCR was performed to determine the gene expression profiles.

Results: No significant difference was observed in particle concentration, size or protein concentration between OA patients and age-matched controls. In the presence of pEVs from OA patients MSC-derived chondrocytes showed a significant increase in the expression of MMP13 (6.1-fold), RUNX2 (1.9-fold) and RANKL (2.3-fold), compared to pEVs from healthy controls. A trend towards higher ADAMTS5 expression (2.5-fold, p=0.0685) with OA pEVs was also observed. Additionally, we found significantly higher expression of WISP1 (24-fold), suggesting activation of Wnt signaling. All other proinflammatory genes tested were not significantly different between the two groups.

Conclusion: A previous study [2] has shown that EVs released from IL-1β stimulated synovial fibroblasts can induce osteoarthritic changes in articular chondrocytes. Here, we show direct evidence that circulating pEVs from OA patients can enhance OA-related genes, like MMP13, in MSC-derived chondrocytes. The expression profile found suggest the presence of Wnt-proteins on pEVs from OA patients, which are known to be involved in cartilage development and we previously have shown that WISP-1 expression is a feature of experimental and human OA [3].

[1] J. Lötvall, et al. Journal of extracellular vesicles (2014).

[2] T. Kato, et al. Arthritis Research & Therapy (2014).

[3] A. B. Blom, et al. Arthritis & Rheumatology (2009).


Disclosure: B. C. H. Pieters, None; O. J. Arntz, None; A. B. Blom, None; P. M. van der Kraan, Contract research UCB, 2; F. A. J. van de Loo, None.

To cite this abstract in AMA style:

Pieters BCH, Arntz OJ, Blom AB, van der Kraan PM, van de Loo FAJ. Chondrocytes Derived from Mesenchymal Stem Cells Differentiated in the Presence of Plasma-Derived Extracellular Vesicles from Osteoarthritic Patients Express Disease-Related Genes [abstract]. Arthritis Rheumatol. 2017; 69 (suppl 10). https://acrabstracts.org/abstract/chondrocytes-derived-from-mesenchymal-stem-cells-differentiated-in-the-presence-of-plasma-derived-extracellular-vesicles-from-osteoarthritic-patients-express-disease-related-genes/. Accessed January 27, 2023.
  • Tweet
  • Email
  • Print

« Back to 2017 ACR/ARHP Annual Meeting

ACR Meeting Abstracts - https://acrabstracts.org/abstract/chondrocytes-derived-from-mesenchymal-stem-cells-differentiated-in-the-presence-of-plasma-derived-extracellular-vesicles-from-osteoarthritic-patients-express-disease-related-genes/

Advanced Search

Your Favorites

You can save and print a list of your favorite abstracts during your browser session by clicking the “Favorite” button at the bottom of any abstract. View your favorites »

ACR Pediatric Rheumatology Symposium 2020

© COPYRIGHT 2023 AMERICAN COLLEGE OF RHEUMATOLOGY

Wiley

  • Home
  • Meetings Archive
  • Advanced Search
  • Meeting Resource Center
  • Online Journal
  • Privacy Policy
  • Permissions Policies
  • Cookie Preferences