Date: Monday, November 9, 2015
Session Type: ACR Poster Session B
Session Time: 9:00AM-11:00AM
To investigate a B-cell subpopulation, exhibiting CD19+CD24-CD38hi phenotype, can be a biomarker for diagnosis and disease activity in IgG4 related disease (IgG4-RD).
Circulating B-cell subsets from 42 untreated IgG4-RD patients, including CD19+CD24-CD38hi, CD19+CD24+CD38-, CD19+CD24intCD38int and CD19+CD24hiCD38hi B cells, were sorted by flow cytometry and their gene expression was measured by microarray. Characterizing of CD19+CD24-CD38hi B cell subset was confirmed by testing surface markers such as CD27, CD95 and HLA-DR, et al. In addition, supernatant IgG4 concentrations were measured by Cytometric Bead Array (CBA).
In untreated IgG4-RD patients, expanded CD19+CD24-CD38hi B-cell subset exhibited a significant different gene expression pattern compared with the other three B cell subsets, which showed much higher expression of Blimp-1 and IRF4, and the lower expression of PAX5 and BCL-6. In addition, CD27, CD95 and HLA-DR were highly expressed on CD19+CD24-CD38hi B-cell subset from IgG4-RD peripheral B cells. Furthermore, CD19+CD24-CD38hi B-cell subset expressed more surface IgG4 and secreted more IgG4. Finally, the level of CD19+CD24-CD38hi B-cells was decreased after therapy, which correlates with disease remission.
Circulating CD19+CD24-CD38hi B-cell subset is elevated in active IgG4-RD, which shows the morphological as well as the phenotypical characteristics of plasmablasts (PB) and decreases during IgG4-RD remission. This B-cell subset might be a potentially useful biomarker for diagnosis and assessing response to treatment.
To cite this abstract in AMA style:Zhang W, Lin W, Chen Y, Yang Y, Chen H, Wu Q, Fei Y, Hu C, Li Y, Zhang X, Zhao Y, Zhang F, Zeng X, Lipsky PE. CD19+CD24-CD38hi B-Cell Subset: A Potential Biomarker for IgG4-Related Disease [abstract]. Arthritis Rheumatol. 2015; 67 (suppl 10). https://acrabstracts.org/abstract/cd19cd24-cd38hi-b-cell-subset-a-potential-biomarker-for-igg4-related-disease/. Accessed October 16, 2021.
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ACR Meeting Abstracts - https://acrabstracts.org/abstract/cd19cd24-cd38hi-b-cell-subset-a-potential-biomarker-for-igg4-related-disease/