ACR Meeting Abstracts

ACR Meeting Abstracts

Abstract Number: 1102

CD19+CD24-CD38hi B-Cell Subset: A Potential Biomarker for IgG4-Related Disease

Wen Zhang1,2, Wei Lin3, Yu Chen4, Yunjiao Yang4, Hua Chen4, QingJun Wu4, Yunyun Fei4, Chaojun Hu4, Yongzhe Li3, Xuan Zhang3, Yan Zhao4, Fengchun Zhang3, Xiaofeng Zeng4 and Peter E. Lipsky5, 1Rheuamtology, Peking Union Medical College Hospital, Beijing, China, 2Peking Union Medical College Hospital, Chinese Academy of Medical Science & Peking Union Medical College, Beijing, China, 3Peking Union Medical College Hospital, Beijing, China, 4Rheumatology, Peking Union Medical College Hospital, Beijing, China, 5Bldg 10 Rm 6d47c, National Institutes of Health, Bethesda, MD

Meeting: 2015 ACR/ARHP Annual Meeting

Date of first publication: September 29, 2015

Keywords: ,

Session Information

Date: Monday, November 9, 2015

Title: B cell Biology and Targets in Rheumatolid Arthritis and other Autoimmune Disease Poster

Session Type: ACR Poster Session B

Session Time: 9:00AM-11:00AM

Background/Purpose:

To investigate a B-cell subpopulation, exhibiting CD19+CD24-CD38hi phenotype, can be a biomarker for diagnosis and disease activity in IgG4 related disease (IgG4-RD).

Methods:

Circulating B-cell subsets from 42 untreated IgG4-RD patients, including CD19+CD24-CD38hi, CD19+CD24+CD38-, CD19+CD24intCD38int and CD19+CD24hiCD38hi B cells, were sorted by flow cytometry and their gene expression was measured by microarray. Characterizing of CD19+CD24-CD38hi B cell subset was confirmed by testing surface markers such as CD27, CD95 and HLA-DR, et al. In addition, supernatant IgG4 concentrations were measured by Cytometric Bead Array (CBA).

Results:

In untreated IgG4-RD patients, expanded CD19+CD24-CD38hi B-cell subset exhibited a significant different gene expression pattern compared with the other three B cell subsets, which showed much higher expression of Blimp-1 and IRF4, and the lower expression of PAX5 and BCL-6. In addition, CD27, CD95 and HLA-DR were highly expressed on CD19+CD24-CD38hi B-cell subset from IgG4-RD peripheral B cells. Furthermore, CD19+CD24-CD38hi B-cell subset expressed more surface IgG4 and secreted more IgG4. Finally, the level of CD19+CD24-CD38hi B-cells was decreased after therapy, which correlates with disease remission.

Conclusion:

Circulating CD19+CD24-CD38hi B-cell subset is elevated in active IgG4-RD, which shows the morphological as well as the phenotypical characteristics of plasmablasts (PB) and decreases during IgG4-RD remission. This B-cell subset might be a potentially useful biomarker for diagnosis and assessing response to treatment.

Disclosure: W. Zhang, None; W. Lin, None; Y. Chen, None; Y. Yang, None; H. Chen, None; Q. Wu, None; Y. Fei, None; C. Hu, None; Y. Li, None; X. Zhang, None; Y. Zhao, None; F. Zhang, None; X. Zeng, None; P. E. Lipsky, None.

To cite this abstract in AMA style:

Zhang W, Lin W, Chen Y, Yang Y, Chen H, Wu Q, Fei Y, Hu C, Li Y, Zhang X, Zhao Y, Zhang F, Zeng X, Lipsky PE. CD19+CD24-CD38hi B-Cell Subset: A Potential Biomarker for IgG4-Related Disease [abstract]. Arthritis Rheumatol. 2015; 67 (suppl 10). https://acrabstracts.org/abstract/cd19cd24-cd38hi-b-cell-subset-a-potential-biomarker-for-igg4-related-disease/. Accessed .

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