ACR Meeting Abstracts

ACR Meeting Abstracts

Abstract Number: 2526

An Autoantibody Profile for The Diagnosis Of Systemic Lupus Erythematosus Using the IMMUNARRAY iCHIP™

Chaim Putterman1, Irene Blanco2, Nicole Jordan3, Yves Renaudineau4, Vered Daniel Carmi5, Rachel Sorek5, Ornit Cohen-Gindi5, Miriam Lerner5, D. Scott Batty5, Idan Tamir5 and Irun R Cohen5,6, 1Division of Rheumatology, Albert Einstein College of Medicine, Bronx, NY, 2Rheumatology, Albert Einstein College of Medicine, Bronx, NY, 3Department of Rheumatology, Albert Einstein College of Medicine, Bronx, NY, 4Unit of immunology, EA 2216, Brest Occidentale University, Brest, France, 5ImmunArray, Rehovot, Israel, 6Immunology, Weizmann Institute of Science, Rehovot, Israel

Meeting: 2013 ACR/ARHP Annual Meeting

Keywords: ,

Session Information

Title: Systemic Lupus Erythematosus-Clinical Aspects III: Biomarkers, Quality of Life and Disease Indicators, Late Complications

Session Type: Abstract Submissions (ACR)

Background/Purpose: Systemic lupus erythematosus (SLE) is a chronic, recurrent, and potentially fatal multisystem inflammatory disorder mainly affecting women. SLE patients produce antibodies to many different self-antigens, some of which play an important role in the pathogenesis of tissue injury in this disease. Accurate and timely diagnosis of SLE is important because early treatment can reduce morbidity and mortality, particularly from lupus nephritis. Nevertheless, currently used serological markers for SLE are lacking in specificity and/or sensitivity. The aim of this study was to develop an improved diagnostic test by measuring and multiplexing specific autoantibody reactivities in SLE patients. 

Methods: Autoantibody reactivity profiles in serum samples collected from 97 SLE patients within three years of the diagnosis were compared with those of 56 healthy controls. In addition, autoantibody profiles of SLE patients was compared with those of patients with progressive systemic sclerosis, primary Sjogren’s syndrome, and primary anti-phospholipid antibody syndrome (APS). Autoantibody profiles were determined using the ImmunArray iCHIP™ – a proprietary protein microarray technology that allows the display of antigens representing a wide range of SLE-associated biochemical pathways on a single chip. 

Results: Using this novel platform, SLE patients could be differentiated from healthy subjects and from patients with systemic sclerosis, Sjogren and APS by a relatively small subset of auto-antigens and Epstein Barr Virus (EBV) antigens. The autoantibody reactivity profile that allowed SLE diagnosis with high sensitivity and specificity displayed differential response to known SLE-specific antigens, such as single-stranded DNA and EBV, and to several novel ones. Validation of the autoantibody profile that differentiates SLE patients from healthy controls in additional patient samples showed good performance (75% sensitivity and 85% specificity).

Conclusion: We successfully developed a novel method of antibody profiling for SLE diagnosis, using a single multiplexed chip.  The ImmunArray iCHIP™ was able to distinguish between lupus patients and healthy controls, as well as between lupus and other inflammatory rheumatic diseases.

Disclosure:

C. Putterman,

Immunarray,

5;

I. Blanco,
None;

N. Jordan,
None;

Y. Renaudineau,
None;

V. Daniel Carmi,

ImmunArray ,

3;

R. Sorek,

ImmunArray,

3;

O. Cohen-Gindi,

ImmunArray,

3;

M. Lerner,

ImmunArray,

3;

D. S. Batty,

ImmunArray,

3;

I. Tamir,

ImmunArray,

3;

I. R. Cohen,

ImmunArray,

5.

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