an Anti-Fima Antibody Attenuates Porphyromonas Gingivalis-Associated Experimental Arthritis

Sang Hoon Jeong¹, Jennifer Lee², Seo Hwa Kim³, Haneul Kim⁴, Seung-Ki Kwok⁵, Sung-Hwan Park⁶ and Ji Hyeon Ju⁷, ¹The Catholic University of Korea, Seoul, Korea, The Republic of, ²Division of Rheumatology, Department of Internal Medicine, College of Medicine, The Catholic University of Korea, Seoul, Korea, Republic of, ³Division of Rheumatology,, Division of Rheumatology, Department of Internal Medicine, College of Medicine, The Catholic University of Korea, Seoul, Korea, The Republic of, ⁴Division of Rheumatology, Department of Internal Medicine, College of Medicine, The Catholic University of Korea, Seoul, Korea, The Republic of, ⁵[email protected], Division of Rheumatology, Department of Internal Medicine, College of Medicine, The Catholic University of Korea, Seoul, South Korea, ⁶Division of Rheumatology, Department of Internal Medicine, College of Medicine, The Catholic University of Korea, Seoul, South Korea, ⁷Division of Rheumatology, Department of Internal Medicine,, College of Medicine, The Catholic University of Korea, Seoul, South Korea

Meeting: 2016 ACR/ARHP Annual Meeting

Date of first publication: September 28, 2016

Keywords: Animal models, Periodontitis and rheumatoid arthritis

Session Information

Date: Sunday, November 13, 2016

Title: Rheumatoid Arthritis – Animal Models - Poster I

Session Type: ACR Poster Session A

Session Time: 9:00AM-11:00AM

Background/Purpose: Evidence suggests that periodontal infection by Porphyromonas gingivalis(Pg) exacerbates rheumatoid arthritis (RA); however, the underlying mechanisms remain unknown. Here, we examined the pathogenic and therapeutic correlation between periodontitis and RA in a mouse model of arthritis.

Methods: Pg strain 2561 was obtained and cultured anaerobically. Collagen-induced arthritis (CIA) mice were infected with Pg or Pg pre-incubated with an anti-FimA antibody (aFimA Ab) specific for fimbriae which are a flexible appendage on the cell surface. Fimbriae plays a pathological role in adhering Pg to gingiva, so neutralizing Pg with aFimA decreases adherence of Pg in mouse cavity. aFimA Ab was cloned and produced by our manufacturing system. Immunohistochemical analysis, microcomputed tomography analysis, confocal microscopy, immunofluorescence analysis, electron microscopy, RT-PCR, and western blot assay were done for proarthritic effect of Pg and neurtralizing anti-arthritic effect of aFimA Ab.

Results: aFimA Ab inhibits Pg induced periodontitis in CIA mice. Pg infection altered the oral microbiota, increased alveolar bone loss, and caused synovitis and joint bone destruction. However, pre-incubation with aFimA Ab led to a significant reduction in the severity of both oral disease and arthritis. aFimA Ab treated Pg fails to trigger arthritis in CIA mice. aFimA Ab treated Pg suppresses cartilage erosion and bone destruction in the joint of CIA mice. Moreover, aFimA Ab attenuated bacteria attachment and aggregation on human gingival and rheumatoid synovial fibroblasts. We discovered bacteria utilized dendritic cells, macrophages, and neutrophils to migrate to the joints of CIA mice.

Conclusion: Pg-induced periodontitis plays a significant role in RA development. Inhibiting Pg adhesion using a FimA Ab prevented RA progression. Disrupting Pg fimbriae suppresses the migration of bacteria to the joints and ameliorates RA.

Disclosure: S. H. Jeong, None; J. Lee, None; S. H. Kim, None; H. Kim, None; S. K. Kwok, None; S. H. Park, None; J. H. Ju, None.

To cite this abstract in AMA style:

Jeong SH, Lee J, Kim SH, Kim H, Kwok SK, Park SH, Ju JH. an Anti-Fima Antibody Attenuates Porphyromonas Gingivalis-Associated Experimental Arthritis [abstract]. Arthritis Rheumatol. 2016; 68 (suppl 10). https://acrabstracts.org/abstract/an-anti-fima-antibody-attenuates-porphyromonas-gingivalis-associated-experimental-arthritis/. Accessed .

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