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Abstract Number: 1465

Absolute Number of Regulatory T Cells Decreased While Osteopontin Increased in Peripheral Blood of New Onset Patients with Rheumatoid Arthritis

Jianfang Xie1, Jiao-Jiao He2, Xiao-Feng Li3 and Chong Gao4, 1the second hospital of shanxi medical university, taiyuan, China, 2The Second Hospital of Shanxi Medical Univerity, Taiyuan, China, 3Rheumatology, the Second Hospital of Shanxi Medical University, Taiyuan, China, 4Department of Pathology, Brigham and Women’s Hospital, Harvard Medical School, Boston, MA, Cambridge, MA

Meeting: 2018 ACR/ARHP Annual Meeting

Keywords: osteopontin and rheumatoid arthritis (RA), T-Regulatory Cells

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Session Information

Date: Monday, October 22, 2018

Session Title: Rheumatoid Arthritis – Diagnosis, Manifestations, and Outcomes Poster II: Diagnosis and Prognosis

Session Type: ACR Poster Session B

Session Time: 9:00AM-11:00AM

Background/Purpose: Rheumatoid arthritis (RA) is a progressive immune-mediated disease involving the synovitis that can culminate in joint destruction and early mortality.IL-17-producing T (Th17) cells and regulatory T (Treg) cells have been showed to play critical roles in the pathogenesis of RA. Osteopontin (OPN) is a matricellular protein that mediates diverse biological functions. OPN mediates cell migration, adhesion, and survival in many cell types. OPN also functions as a Th1 cytokine, promotes cell-mediated immune responses, and plays a role in chronic inflammatory and autoimmune diseases. Recently, studies revealed that OPN produced in RA SF was responsible for markedly increased Th17 and Both Treg-intrinsic and Treg-extrinsicis factors maintaining Bcl6 are important for controlling Treg cell stability. Recently, our studies have found that absolute number of CD4+CD25+Foxp3+ regulatory T cells (CD4+Tregs) was significantly decreased in peripheral blood from patients with RA as compared to that from normal controls. The purpose is to investigate the absolute number of Th17, CD4+CD25+Foxp3+ Treg cells as well as OPN content in peripheral blood of patients with RA and the correlation between OPN content with Th17 and Treg cells, to find a possible pathogenesis related to RA and new therapy for the RA treatment.

Methods: A total 67 of new-onset diagnosed patients with RA fulfilled the 2010 ACR/EULAR Classification Criteria of Rheumatoid Arthritis were enrolled and 93 healthy donors were used as control. The absolute number of peripheral CD4+Tregs cell was detected by multicolor flow cytometry . ELISA was used to detect OPN. Meanwhile, Test parameters were anti-cyclic citrullinated peptide antibody (Anti-CCP), erythrocyte sedimentation rate (ESR), C reactive protein (CRP), rheumatoid factor(RF). At the same time, the DAS28 score of RA group was calculated.

Results: The levels of OPN in RA patients were increased significantly [(14.84±8.64) vs (10.59±3.40), P<0.001] and significantly positively correlated to ESR, DAS28 score, and RF (r=0.284, P=0.02; r=0.290, P=0.039; r=0.376, P=0.007). The absolute number of Treg cells was significantly decreased in RA patients as compared with the controls[(23.63±2.80) vs (33.67±26.83)ng/µl, P<0.001]. However, the absolute number of Treg cells was negatively to OPN levels, and the difference was statistically significant (r=-0.459, P=0.000). The absolute number of Th17 cells tends to reduce and there was no statistical significance[(7.64±6.44 ) vs (6.64±4.26) ng/µl, P=0.0129]. There is no significant correlation between OPN and Th17(r=0.006,P=0.963).

Conclusion: The levels of OPN in peripheral blood plasma in RA were significantly higher than in healthy group, OPN was positively correlated with the laboratory parameters of disease activity. There was a negative correlation between Treg cells and OPN in RA group, which suggested that the reduction of Treg cell levels may be associated with OPN concentration. The correlation of decreased CD4+Tregs and OPN may be involved in the pathogenesis of RA. Whether OPN lays a role in the reduction of Treg cells need to study further


Disclosure: J. Xie, None; J. J. He, None; X. F. Li, None; C. Gao, None.

To cite this abstract in AMA style:

Xie J, He JJ, Li XF, Gao C. Absolute Number of Regulatory T Cells Decreased While Osteopontin Increased in Peripheral Blood of New Onset Patients with Rheumatoid Arthritis [abstract]. Arthritis Rheumatol. 2018; 70 (suppl 10). https://acrabstracts.org/abstract/absolute-number-of-regulatory-t-cells-decreased-while-osteopontin-increased-in-peripheral-blood-of-new-onset-patients-with-rheumatoid-arthritis/. Accessed January 27, 2021.
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