ACR Meeting Abstracts

ACR Meeting Abstracts

Abstract Number: 786

Distinguishing S100 Proteins and Cytokine Levels Between Active and Inactive Uveitis in Children with Juvenile Idiopathic Arthritis

Jackeline Rodriguez-Smith1, Virginia Utz 2, Sherry Thornton 1, Grant Schulert 1, Adam Kauffman 3, Alyssa Sproles 1, Najima Mwase 1, Theresa Hennard 4, Alexei A. Grom 1, Mekibib Altaye 5, Gary Holland 6 and Sheila Angeles-Han 7, 1Division of Rheumatology, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, 2Division of Ophthalmology, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, 3Cincinnati Eye Institute and Cincinnati Children's Hospital Medical Center, Cincinnati, OH, 4Division of Rheumatology, Cincinnati Children's Hospital and Medical Center, Cincinnati, OH, 5Divison of Biostatistics and Epidemiology and Department of Pediatrics, University of Cincinnati, Cincinnati, OH, 6UCLA Stein Eye Institute and David Geffen School of Medicine at University of California, Los Angeles, Ca, Los Angeles, CA, 7Division of Rheumatology, Cincinnati Children's Hospital Medical Center, Cinicinnati

Meeting: 2019 ACR/ARP Annual Meeting

Keywords: , , ,

Session Information

Date: Sunday, November 10, 2019

Title: Pediatric Rheumatology – ePoster I: Basic Science, Biomarkers, & Sclerodermic Fever

Session Type: Poster Session (Sunday)

Session Time: 9:00AM-11:00AM

Background/Purpose: Uveitis occurs in 10-20% of children with Juvenile Idiopathic Arthritis (JIA) and is typically asymptomatic. Ocular complications occur in 50% of children, (i.e. cataracts, vision loss). Even after uveitis is controlled, risk of disease exacerbation still exists. Therefore, frequent ophthalmic screening and monitoring is important for detection and management of JIA-associated uveitis (JIA-U). Potential objective measures of ocular inflammation are S100 proteins, cytokines, and chemokines that have been identified as biomarkers in aqueous humor.  However, aqueous humor collection is invasive. Measuring the same biomarkers in tears, would be a less invasive approach. Our objective is to determine whether S100 proteins, cytokines, and chemokine levels differ in tears of JIA-U children with active or inactive uveitis.

Methods: Tears were collected using Schirmer strips from children ≥5 years old with JIA-U (n=20) and pediatric healthy controls (n=20) during routine clinic visit. S100A8, A9, and A12 were measured by ELISA, and IL-18, IL-8, IP-10, MCP-1, RANTES, and sICAM-1 by Luminex assays. Levels were compared between JIA-U children and healthy controls and between JIA-U children with active and inactive uveitis.

Results: S100 proteins, cytokines and most chemokines levels were similar in JIA-U compared to controls. However, differences were observed between JIA-U patients with active and inactive uveitis. Patients with active uveitis had significantly higher levels of tear S100A12 as compared to inactive uveitis (mean 27,722 pg/ML [SE 1.3] vs. 5,937pg/ML [SE 1.3], p=0.0001). Similar increased levels in active uveitis as compared to inactive uveitis for IL-8 (73 pg/ML [SE 1.3] vs. 37 pg/ML [SE 1.1], p = 0.026) and sICAM-1 (15,823 pg/ML [SE 1.2] vs. 8,778 pg/ML [SE 1.6], p=0.004)

Conclusion: Identifying uveitis biomarkers using tears would provide a noninvasive and objective method of detecting and monitoring uveitis. Our results indicate that S100A12, IL-8 and sICAM-1 from tears could be utilized as potential biomarkers for distinguishing inactive and active uveitis. This suggests that neutrophils may play a role in the pathogenesis of anterior uveitis which has been reported in an animal model of acute anterior uveitis. 

Disclosure: J. Rodriguez-Smith, None; V. Utz, Retrophin, 2; S. Thornton, None; G. Schulert, Novartis, 5, 8; A. Kauffman, 1800 contacts and alcon, 5; A. Sproles, None; N. Mwase, None; T. Hennard, None; A. Grom, AB2 Bio Ltd, 2, 5, AB2Bio, 2, 5, Children's Hospital Medical Center, 3, Novartis, 2, 5, Novartis Pharmaceuticals Corporation, 2, 5, Novartis Pharmaceuticals Corporations, 5, NovImmune, 2, 5, Novimmune, 2, 5; M. Altaye, None; G. Holland, None; S. Angeles-Han, None.

To cite this abstract in AMA style:

Rodriguez-Smith J, Utz V, Thornton S, Schulert G, Kauffman A, Sproles A, Mwase N, Hennard T, Grom A, Altaye M, Holland G, Angeles-Han S. Distinguishing S100 Proteins and Cytokine Levels Between Active and Inactive Uveitis in Children with Juvenile Idiopathic Arthritis [abstract]. Arthritis Rheumatol. 2019; 71 (suppl 10). https://acrabstracts.org/abstract/distinguishing-s100-proteins-and-cytokine-levels-between-active-and-inactive-uveitis-in-children-with-juvenile-idiopathic-arthritis/. Accessed .

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