ACR Meeting Abstracts

ACR Meeting Abstracts

Abstract Number: 5

Short Chain Fatty Acid Acetate Induces Regulatory B Cells in Mice and Humans, Which Can Protect Against Arthritis

Claire I. Daien1,2,3, Julie Mielle1,4, Rachel Audo1,2,5, Lake-Ee Quek1, James Krycer1, Gauthier Rathat6, Gabriela Pinget1, Sumaiya Hoque7, Charles Mackay8, Jian Tan9 and Laurence Macia1, 1Charles Perkins Centre, The University of Sydney, Sydney, Australia, 2IGMM, CNRS UMR5535, Montpellier, Montpellier, France, 3Department of rheumatology, Lapeyronie Hospital and Montpellier University, Montpellier, France, 4IGMM CNRS UMR5535, Montpellier, France, 5Rheumatology, Lapeyronie Hospital and Montpellier University, Montpellier, France, 6Arnaud-de-Villeneuve Hospital, Montpellier, France, 7Charles Perkins Centre, The University of Sydney, Sydney, France, 8Monash University, Melbourne, Australia, 9Charles Perkins Centre, The University of Sydney, Montpellier, Australia

Meeting: 2018 ACR/ARHP Annual Meeting

Keywords: , , ,

Session Information

Date: Sunday, October 21, 2018

Title: B Cell Biology and Targets in Autoimmune and Inflammatory Disease Poster

Session Type: ACR Poster Session A

Session Time: 9:00AM-11:00AM

Background/Purpose:

Autoimmunity and more broadly non-communicable diseases develop with the imbalance between anti- and pro-inflammatory immune cell subsets, leading to an uncontrolled and damaging inflammation. Evidences have shown that short chain fatty acids promote anti-inflammatory regulatory T cell protecting from type 1 diabetes, colitis and allergies in mice. In the present study, we investigated the benefits of short chain fatty acid acetate extend to other immune cells involved in tolerance, the regulatory B cells (Breg).

Methods:

For this purpose, we assessed the effect of acetate on Breg in vitro and in vivo through oral supplementation or intraperitoneal injections. The functions of acetate-induced Bregs were assessed i) in vitro using co-culture with naïve T cells to assess Th17 differenciation ii) in vivo with adoptive transfer of acetate-stimulated B cells in a model of collagen antibody-induced arthritis (CAIA). In addition, the effect of acetate was also evaluated on B cells from human samples.

Results:

We showed that both in vivo and in vitro acetate promoted Breg differentiation from B1a cells but not from B2 cells in mice. Acetate-induced Breg cells could protect from collagen antibody-induced arthritis development when adoptively transferred. These effects were neither through specific G-protein receptor activation nor HDAC inhibition but by inducing metabolic changes particularly in B1a cells by fueling TCA cycle. Similarly, we found that acetate also promotes human Breg cells through metabolic changes.

Conclusion:

This work suggest that acetate might be a promising therapeutic approach to restore Breg population in autoimmune diseases such as Rheumatoid Arthritis in which they are defective.

Disclosure: C. I. Daien, None; J. Mielle, None; R. Audo, None; L. E. Quek, None; J. Krycer, None; G. Rathat, None; G. Pinget, None; S. Hoque, None; C. Mackay, None; J. Tan, None; L. Macia, None.

To cite this abstract in AMA style:

Daien CI, Mielle J, Audo R, Quek LE, Krycer J, Rathat G, Pinget G, Hoque S, Mackay C, Tan J, Macia L. Short Chain Fatty Acid Acetate Induces Regulatory B Cells in Mice and Humans, Which Can Protect Against Arthritis [abstract]. Arthritis Rheumatol. 2018; 70 (suppl 9). https://acrabstracts.org/abstract/short-chain-fatty-acid-acetate-induces-regulatory-b-cells-in-mice-and-humans-which-can-protect-against-arthritis/. Accessed .

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