Abstracts tagged "Enzyme-Linked Immunoabsorbant Assays (ELISA)"

Abstract Number: 130 • 2019 ACR/ARP Annual Meeting
Is There Clinically Relevant Plasma Interference with ELISA Detection of APS Antibodies? Reproducibility of Serum and Plasma Testing in a Real-World Clinical Setting
Michael Pham¹, Giovanni Orsolini ², Cynthia Crowson ³, Melissa Snyder ¹, Rajiv Pruthi ⁴ and Kevin Moder ⁵, ¹Mayo Clinic, Rochester, MN, ²University of Verona, Verona, Italy, ³Mayo Clinic Rochester, Rochester, ⁴Mayo Clinic, Rochester, ⁵Mayo Clinic Rochester, rochester, MN
Background/Purpose: Traditionally in ELISA detection of APS antibodies, the use of serum is thought to be preferable over plasma according to international consensus bodies. The…
Abstract Number: 491 • 2019 ACR/ARP Annual Meeting
Diagnostic Performance of Anti-cyclic Citrullinated Peptide (CCP) 2 and CCP3.1 Assays in Early Rheumatoid Arthritis
Daniela Sieghart¹, Christian Konrad ², Sascha Swiniarski ², Helmuth Haslacher ³, Daniel Aletaha ⁴ and Günter Steiner ¹, ¹Division of Rheumatology, Department of Internal Medicine III, Medical University of Vienna, Vienna, Austria; Ludwig Boltzmann Institute for Arthritis and Rehabilitation, Vienna, Austria, Vienna, Austria, ²Thermo Fisher Scientific, Freiburg, Germany, Freiburg, Germany, ³Department of Laboratory Medicine, Medical University of Vienna, Vienna, Austria, Vienna, Austria, ⁴Medical University of Vienna, Vienna, Austria
Background/Purpose: Anti-cyclic citrullinated peptide (CCP) antibodies are the most specific markers for rheumatoid arthritis (RA). Different generations of assays (CCP1-CCP3) have been developed which show…
Abstract Number: 661 • 2019 ACR/ARP Annual Meeting
Association of Serum and Urine Levels of TWEAK, MCP-1 and NGAL with Disease Activity in Systemic Lupus Erythematosus
SAFAK MIRIOGLU ¹, SUZAN CINAR ², Halil Yazici ³, Ahmet Gül ⁴, Lale Öcal ⁴, Murat Inanc ⁴ and Bahar Artim-Esen⁴, ¹Istanbul University, Istanbul Faculty of Medicine, Department of Internal Medicine, ISTANBUL, Turkey, ²Istanbul University,Department of Immunology, Institute of Experimental Medicine, ISTANBUL, Turkey, ³Istanbul University, Istanbul Faculty of Medicine, Department of Internal Medicine, Division of Nephrology, Istanbul, Turkey, ⁴Istanbul University, Istanbul Faculty of Medicine, Department of Internal Medicine, Division of Rheumatology, Istanbul, Turkey
Background/Purpose: TWEAK, MCP-1 and NGAL, mediators in pathogenesis of systemic lupus erythematosus (SLE), are proinflammatory cytokines/chemokines that are thought as potential biomarkers reflecting disease activity.…
Abstract Number: 2642 • 2019 ACR/ARP Annual Meeting
ANCA Testing: Final Diagnoses in Cases with Positive Immunofluorescence and Negative ELISA
‪Gabriel Breuer‬‏¹, Bashar Fteiha ¹, Alon Benaya ¹, Marwan Abu Sneineh‬‏ ¹ and Gideon Nesher ¹, ¹Shaare Zedek Medical Center, Jerusalem, Yerushalayim, Israel
Background/Purpose: With the widespread availability of anti-neutrophil cytoplasmic antibody (ANCA) testing, interpreting positive results has become increasingly challenging. In addition to ANCA Associated Vasculitis (AAV),…
Abstract Number: 596 • 2016 ACR/ARHP Annual Meeting
Association Between Plasma Certolizumab Pegol Concentration and Improvement in Disease Activity in Rheumatoid Arthritis and Crohn’s Disease
Gerrit Wolbink^1,2, Philippe Goupille³, William Sandborn⁴, Hubert Marotte⁵, Denis Mulleman³, David Ternant³, Stéphane Paul⁵, Marc de Longueville⁶, Niels Vande Casteele^4,7, Miren Zamacona⁶, Cathy O'Brien⁶, Tore K. Kvien⁸ and Arthur F. Kavanaugh⁹, ¹Amsterdam Rheumatology Immunology Center (ARC), Reade, Amsterdam, Netherlands, ²Department of Immunopathology, Sanquin, Amsterdam, Netherlands, ³Université François-Rabelais, Tours, France, ⁴Division of Gastroenterology, UC San Diego School of Medicine, La Jolla, CA, ⁵Centre Hospitalier Universitaire de Saint-Étienne, Saint-Étienne, France, ⁶UCB Pharma, Brussels, Belgium, ⁷KU Leuven Department of Pharmaceutical and Pharmacological Sciences, Leuven, Belgium, ⁸Department of Rheumatology, Diakonhjemmet Hospital, Oslo, Norway, ⁹Division of Rheumatology, Allergy & Immunology, UC San Diego School of Medicine, La Jolla, CA
Background/Purpose: Anti-TNFs neutralize the TNF-mediated component of inflammatory diseases such as rheumatoid arthritis (RA) and Crohn’s disease (CD) to reduce disease activity. In this study,…
Abstract Number: 1073 • 2016 ACR/ARHP Annual Meeting
Antiphospholipid Syndrome Alliance for Clinical Trials & International Networking (APS ACTION) Clinical Database and Repository Analysis: The Comparison of Real World and Core Laboratory Antiphospholipid Antibody ELISA Results
Savino Sciascia¹, Rohan Willis², Vittorio Pengo³, Steven Krilis⁴, Danieli Andrade⁵, Doruk Erkan⁶, Maria Laura Bertolaccini⁷ and On Behalf of APS ACTION .⁸, ¹Department of Rare, Immunologic, Hematologic and Immunohematologic Diseases, Centro di Immunopatologia e Documentazione su Malattie rare, Torino, Italy, ²Rheumatology/Dept Int Med, University of Texas Medical Branch, Galveston, TX, ³Azienda Ospedaliera of Padova, University of Padova, Padova, Italy, ⁴Department of Immunology, Allergy and Infectious Diseases, University of New South Wales, Sydney, Australia, ⁵Rheumatology, University of Sao Paulo, Sao Paulo, Brazil, ⁶Rheumatology, Hospital for Special Surgery- Weill Cornell Medicine, New York, NY, ⁷Academic Department of Vascular Surgery, King’s College London, London, United Kingdom, ⁸., New York, NY
Background/Purpose: The APS ACTION International Clinical Database and Repository (“Registry”) was created to study the natural course of disease over 10 years in persistently aPL-positive…
Abstract Number: 2589 • 2016 ACR/ARHP Annual Meeting
Comparison of Two Enzyme-Linked Immunosorbent Assays Used for Drug Concentration Monitoring in Psoriatic Arthritis Patients Treated with Certolizumab Pegol
Stéphane Paul¹, John Smeraglia², Marc de Longueville², Cathy O'Brien² and Ermis Parussini³, ¹Centre Hospitalier Universitaire de Saint-Étienne, Saint-Étienne, France, ²UCB Pharma, Brussels, Belgium, ³Theradiag, Croissy-Beaubourg, France
Background/Purpose: Measurement of plasma anti-TNF concentration, in combination with the assessment of clinical outcomes, may be used to optimize dosing regimens for individual patients (pts).…
Abstract Number: 821 • 2015 ACR/ARHP Annual Meeting
Elevated Serum Levels of Soluble CD146 in Patients with Systemic Sclerosis
Kazunori Kato¹, Tomoko Ito², Naoto Tamura², Sayuri Okuda¹, Masakazu Matsushita², Kurisu Tada², Ken Yamaji² and Yoshinari Takasaki², ¹Department of Biomedical Engineering, Toyo University, Saitama, Japan, ²Department of Internal Medicine and Rheumatology, Juntendo University School of Medicine, Tokyo, Japan
Background/Purpose: CD146 is a transmembrane glycoprotein belonging to Ig-superfamily. Human endothelial cells, activated fibroblasts and Th17 cells express CD146 that involved in angiogenesis, fibrosis and…
Abstract Number: 1258 • 2014 ACR/ARHP Annual Meeting
Enzyme-Linked Immunosorbent Assays for Detection of Anti-Transcriptional Intermediary Factor-1 Gamma and Anti-Mi-2 Autoantibodies in Dermatomyositis: Utility and Crossreactivity
Manabu Fujimoto¹, Akihiro Murakami², Shunsuke Kurei², Akiko Kuwajima³, Yasuhiro Fujisawa¹, Atsushi Kawakami⁴, Michiaki Mishima⁵, Shinji Sato⁶, Mariko Seishima⁷, Takafumi Suda⁸, Tsuneyo Mimori⁹, Kazuhiko Takehara¹⁰ and Masataka Kuwana¹¹, ¹Department of Dermatology, Faculty of Medicine, University of Tsukuba, Tsukuba, Japan, ²Medical & Biological Laboratories Co., Ltd, Nagaya, Japan, ³Medical & Biological Laboratories Co.,Ltd., Nagoya, Japan, ⁴Department of Immunology and Rheumatology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan, ⁵Department of Respiratory Medicine, Graduate School of Medicine, Kyoto University, Kyoto, Japan, Kyoto, Japan, ⁶Division of Rheumatology, Department of Internal Medicine, Tokai University School of Medicine, Isehara, Japan, ⁷Department of Dermatology, Gifu University Graduate School of Medicine, Gifu, Japan, ⁸Second Division, Department of Internal Medicine, Hamamatsu University School of Medicine, Hamamatsu, Japan, ⁹Dept of Rheum & Clinical Immunology, Kyoto Univ Grad Schl of Med, Kyoto, Japan, ¹⁰Dermatology, Kanazawa University, Kanazawa, Japan, ¹¹Division of Rheumatology, Department of Internal Medicine, Keio University School of Medicine, Tokyo, Japan
Background/Purpose Anti-transcriptional intermediary factor 1 (TIF-1) and anti-Mi-2 antibodies (Abs) are myositis-specific autoantibodies (MSA) selectively detected in dernatomyositis (DM) patients. Anti-TIF-1 Ab is frequently found…
Abstract Number: 648 • 2013 ACR/ARHP Annual Meeting
A Possible Contribution of Inducible Costimulator to the Development of Skin Sclerosis and Interstitial Lung Disease in Systemic Sclerosis
Koichi Yanaba¹, Yoshihide Asano² and Shinichi Sato², ¹Dermatology, The University of Tokyo, Tokyo, Japan, ²Dermatology, University of Tokyo Graduate School of Medicine, Tokyo, Japan
Background/Purpose: Systemic sclerosis (SSc) is a heterogeneous disorder characterized by excessive fibrosis and microvascular damage of the skin and various internal organs. Cutaneous mononuclear cell…
Abstract Number: 26 • 2013 ACR/ARHP Annual Meeting
Autoantibodies Directed Against Cell Surface Components In Autoimmune Disease Patients: Proposal Of a Novel ELISA For The Detection Of Anti-Endothelial Cell Antibodies
Keiji Miura¹, Ayako Kondo², Kazuo Takahashi², Daisuke Hirano², Yoshiyuki Hiki³, Shunji Yoshida², Yukio Yuzawa² and Yoshikazu Kurosawa³, ¹Institute for Comprehensive Medical Science, Fujita Health Universtiy, Toyoake, Japan, ²Fujita Health Universtiy School of Medicine, Toyoake, Japan, ³Fujita Health Universtiy, Toyoake, Japan
Background/Purpose: Sera from patients with systemic vasculitis or inflammatory conditions have been reported to contain antibodies that bind to endothelial cells (EC), i.e., AECA (anti-endothelial…
Abstract Number: 1536 • 2012 ACR/ARHP Annual Meeting
Clinical Features of Patients with Anti-Neutrophil Cytoplasmic Autoantibodies Targeting Native Myeloperoxidase Antigen
Yuji Yamanishi¹, Toshiko Ito-Ihara², Shigeto Kobayashi³, Peter Y. Shane⁴, Gary S. Firestein⁵, Hiroshi Hashimoto⁶ and Kazuo Suzuki⁷, ¹Division of Rheumatology, Hiroshima Rheumatology Clinic, Hiroshima, Japan, ²Department of Clinical Innovative Medicine, Translational Research Center, Kyoto University Hospital, Kyoto, Japan, ³Department of Internal Medicine, Juntendo University Koshigaya Hospital, Tokyo, Japan, ⁴Tokyo Medical and Dental University, Tokyo, Japan, ⁵Div of Rheumatology, UCSD School of Medicine, La Jolla, CA, ⁶Juntendo Tokyo Koto Geriatric Center, Tokyo, Japan, ⁷National Institute of Infectious Diseases, Tokyo, Japan
Background/Purpose: ANCA is a useful diagnostic marker in systemic vasculitic disorders with small-vessel involvement, but depending on the particular test used the myeloperoxidase (MPO)-ANCA results…