Session Information
Session Type: Abstract Submissions (ACR)
Background/Purpose: Psoriatic arthritis (PsA) is an inflammatory joint disease associated with psoriasis. There is little understanding of the relative levels of cytokines and chemokines between the skin and synovium in PsA. The purpose of this study was to better define inflammatory pathways in paired lesional skin and affected synovial tissue in patients with psoriatic arthritis. We conducted a comprehensive cytokine profile in psoriatic skin and joint disease.
Methods: RT-PCR was performed using coupled directed pre-amplification technique.
Results: Of all the cytokines evaluated, only Th17- associated cytokines (IL17A, IL17F) and IL1a were significantly increased in psoriatic skin when compared to affected synovium. IL6 was significantly increased in synovium. Th1- (IFNg) and Th2- (IL4, IL13) associated cytokines were not significantly different. Th9-(IL9), Th22- (IL22), and Treg- (FOXP3) associated cytokines, as well as IL1b, IL2, IL2RA, TNFa and IL8, were also not significantly different. There was no correlation of gene expression levels between pairs of skin and synovium from the same subject.
Conclusion: This is the first comprehensive molecular comparison of paired lesional psoriatic skin and affected synovium in psoriatic arthritis. This is also the first study to investigate IL9 in psoriatic arthritis tissue. These results demonstrate that there are many shared cytokines and chemokines between matched pairs of lesional psoriatic skin and synovium from inflamed joints in patients with PsA. However, IL1a, IL6, IL17A and IL17F were significantly different. No correlation between gene products was noted between paired samples of synovium and skin, suggesting that there are distinct immunological phenotypes for synovium and skin within the same person. Therefore, the pathomechanisms of these two symptomatic organs may be at least partially independent from one another. This could have implications for the direction of future therapies.
Disclosure:
J. Belasco,
None;
H. Mitsui,
None;
M. Suarez-Farinas,
Idera Pharmaceuticals,
2;
J. S. Louie,
Amgen,
8,
Genentech and Biogen IDEC Inc.,
8,
Pfizer Inc,
8;
N. Wei,
None;
N. Gulati,
None;
J. G. Krueger,
Amgen,
9,
Biogen Idec,
9,
Boehringer Ingelheim,
9,
Centocor, Inc.,
9,
Lilly,
9,
Novartis Pharmaceutical Corporation,
9,
Pfizer Inc,
9.
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ACR Meeting Abstracts - https://acrabstracts.org/abstract/upregulated-th17-and-innate-pathways-are-more-characteristic-of-the-skin-than-the-synovium-in-psoriatic-arthritis/