Session Type: ACR Poster Session B
Session Time: 9:00AM-11:00AM
Background/Purpose: Vδ2T cells, a subpopulation of γδ T cells with inflammatory features, play a vital role in inflammation, tumor immunology, infectious disease and autoimmunity. However, the role of Vδ2 T cells in the pathogenesis of rheumatoid arthritis remains elusive.
Methods: 68 patients with rheumatoid arthritis, 21 patients with osteoarthritis and 21 healthy controls were enrolled in the study. All RA patients fulfilling the 2010 ACR/EULAR criteria for RA. Peripheral Vδ2T population, apoptosis, proliferation, chemokine receptor expression and pro-inflammatory cytokine secretion were quantified by flow cytometry. The infiltration of Vδ2 T cells within synovium was examined by immunohistochemistry and flow cytometry. The effect of TNF-α and IL-6 on Vδ2 T migration was determined by flow cytometry and transwell migration assay.
Results: The percentage of peripheral Vδ2T cells of active RA were significantly decreased compared with healthy controls (1.80±1.76% vs 5.68±2.72%), which were negatively correlated with the disease activity indexes including DAS28(r=-0.6341, p<0.01), CRP(r=-0.4352, p<0.01) and ESR(r=-0.4364, p<0.01). However, the Vδ2T cells infiltrated in the synovium of RA were increased compared with OA (p<0.05). Comparing with OA Vδ2T cells, both peripheral and synovium Vδ2T cells of RA produced higher level of IFN-γ and IL-17 (p<0.05). The chemokine receptor CCR5 and CXCR3 expressed on Vδ2T cells in RA were significantly higher than HC and OA patients (p<0.05), which were induced by TNF-α and IL-6. TNF-α antagonist therapy restored the peripheral Vδ2 T cell in RA.
Conclusion: Elevated TNF-α and IL-6 in RA patients induced high expression of CCR5 and CXCR3 on Vδ2T cells, which subsequently promote Vδ2 T cells infiltrate into synovium and play an important role in the pathogenesis of RA. Vδ2 T cell is a promising potential biomarker and therapeutic target of RA.
To cite this abstract in AMA style:MO W, YIN S, ZHOU C, Zhang X. TNF-α and IL-6 Induced Upregulation of CCR5 and CXCR3 Participate in Vδ2 Chemotaxis in RA [abstract]. Arthritis Rheumatol. 2016; 68 (suppl 10). https://acrabstracts.org/abstract/tnf-%ce%b1-and-il-6-induced-upregulation-of-ccr5-and-cxcr3-participate-in-v%ce%b42-chemotaxis-in-ra/. Accessed February 25, 2020.
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