Session Information
Date: Sunday, October 21, 2018
Title: Systemic Lupus Erythematosus – Clinical Poster I: Clinical Manifestations and Comorbidity
Session Type: ACR Poster Session A
Session Time: 9:00AM-11:00AM
Background/Purpose:
Micro-RNAs play an important role in regulating gene expression at the posttranscriptional level. Recent data have shown that microRNAs are critical for the development and function of immune system, both innate and adaptive immunity.(1,2) MicroRNAs are differentially expressed in patients with systemic lupus erythematosus (SLE), especially in association with lupus nephritis.(3,4) The heterogeneity of patient ethnicity and variety in detection method may in part explain some of the discrepancies of positive micro-RNA list in SLE patients in different studies. Testing serum or urine microRNA expression would offer a promising tool to illustrate the pathogenesis of SLE, provide novel biomarkers and potential therapeutic soption.(5)
Methods:
Expression levels of micro-RNA-142-3p extracted from peripheral blood mono-nuclear cells determined using quantitative reverse transcription–polymerase chain reaction assay. A total of 90 plasma samples were obtained from 30 SLE patients without clinical and laboratory evidence of lupus nephritis, 30 SLE patients with lupus nephritis and 30 healthy control subjects.
Results:
The expression of micro-RNA-142-3p in SLE patients was significantly lower than the expression in normal healthy control, p<0.001.In addition the Roc curve of micro-RNA-142-3p showed that micro-RNA-142-3p expression levels can significantly discriminate between lupus patients with and without lupus nephritis at a cut off level ≤5.7×10-6 with a sensitivity of 83.33% and specificity of 90% .We also found a significant correlation between micro-RNA-142-3p expression levels and the pathological activity index of renal biopsy, while there was no significant correlation between micro-RNA-142-3p expression level and the pathological chronicity index.
Conclusion:
The expression level of micro-RNA-142-3p could be considered a diagnostic marker of SLE. Also, the expression level of micro-RNA-142-3p could be considered a potential biomarker for recognition of renal involvement in SLE patients.
References : 1. Mehta A, Baltimore D. MicroRNAs as regulatory elements in immune system logic. Nat Rev Immunol 2016;16:279-94.
2. Liu J, Qian C, Cao X. Post-translational modification control of innate immunity. Immunity 2016; 45:15–30.
3. Dai Y, Huang YS, Tang M, Lv TY, Hu CX, Tan YH, et al. Microarray analysis of microRNA expression in peripheral blood cells of systemic lupus erythematosus patients. Lupus 2007;16 :939–46.
4. Te JL, Dozmorov IM, Guthridge JM, Nguyen KL, Cavett JW, Kelly JA, et al. Identification of unique microRNA signature associated with lupus nephritis. PLoS One 2010;5(5): e10344.
5. Wang Z, Chang C, Peng M, Lu Q. Translating epigenetics into clinic: focus on lupus. Clin Epigenetics 2017; 9:78.
To cite this abstract in AMA style:
Elkhalifa M, Zehairy M, Tayel M, Elkeraie A, Elkaffash D, Baddour N. The Role of Micro-RNA 142-3p Expression in Lupus Nephritis in an Egyptian Cohort [abstract]. Arthritis Rheumatol. 2018; 70 (suppl 9). https://acrabstracts.org/abstract/the-role-of-micro-rna-142-3p-expression-in-lupus-nephritis-in-an-egyptian-cohort/. Accessed .« Back to 2018 ACR/ARHP Annual Meeting
ACR Meeting Abstracts - https://acrabstracts.org/abstract/the-role-of-micro-rna-142-3p-expression-in-lupus-nephritis-in-an-egyptian-cohort/