Session Information
Session Type: Abstract Submissions (ACR)
Background/Purpose:
Today, there is no treatment to cure osteoarthritis (OA) or to delay effectively its progression. Current treatments are mainly based on the alleviation of painful symptoms but are unable to restore the cartilage. The development of biomaterial is a promising approach. Herein, we report the evaluation of a new biomaterial composed of alginate-chitosan (AC) beads dispersed in an (H) hydrogel derived from chitosan on OA rabbit model.
Methods:
OA was surgically induced by the transection of the anterior cruciate ligament (ACLT) in HYLA albino rabbits. One week after surgery, animals were randomly divided into 3 groups: group I (n=7): mix of AC beads and H hydrogel; group II (n=7): H hydrogel alone; group III (n=7): saline solution (control). The treatments (900 µl) were injected intra-articularly. X-rays from the right knee were performed before surgery, at the time of injection and at sacrifice. The standard radiographs were acquired in extension and scored by the Kellgren and Lawrence (K&L) scale. After 6 weeks, animals were euthanized and the right joint was dissected. The macroscopic evaluation of cartilage from femoral condyles and tibial plateaus stained with India ink was done. Histological sections from bearing areas of each compartment and synovial membrane were stained with Safranine-O/fast green or hematoxylin/eosin. Histological evaluation was done according to the OARSI histological score where 0 represented a normal situation and 24 points the maximum severity score. Blood samples were collected the day of injection and prior the sacrifice. Prostaglandin E2 (PGE2) and C-reactive protein (CRP) were measured in serum using immunoassays.
Results:
The X-rays analysis showed a significant decrease (p <0.05) of the K&L score in group I (AC beads and H hydrogel; 1.5 ± 0.2) compared with group II (H hydrogel; 2.2 ± 0.5) and group III (saline solution; 3.0 ± 0.4). The size and the severity of the macroscopic OA cartilage lesion tended to decrease in group I compared to the other groups. The histological global score that refers to all compartments of the knee joint was significantly decreased in group I (11.0 ± 0.7) compared to group II (14.4 ± 0.6, p <0.01) and group III (14.8 ± 0.6, p <0.001). The injection of AC beads and H hydrogel also tended to reduce the synovial membrane inflammation. No significant variation of PGE2 and CRP serum levels were observed in each after 6 weeks follow-up whatever the treatment injected.
Conclusion:
Alginate-chitosan beads dispersed in H hydrogel prevented OA in rabbit after ACL transection. This effect was not observed with the hydrogel alone, suggesting that AC beads play a role in joint protection. The preventive effect was observed in all joint compartments indicating a global protective effect of this new injectable biomaterial.
Disclosure:
F. Oprenyeszk,
None;
M. Chausson,
None;
V. Maquet,
None;
J. E. Dubuc,
None;
Y. Henrotin,
None.
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ACR Meeting Abstracts - https://acrabstracts.org/abstract/protective-effect-of-intra-articular-injection-of-alginate-chitosan-beads-in-an-hydrogel-against-the-development-of-experimental-osteoarthritis-lesions-in-rabbit/