Session Title: Cytokines, Mediators, and Gene Regulation
Session Type: Abstract Submissions (ACR)
Background/Purpose: IL-36α is a recent described IL-1 cytokine family member with proinflammatory and clear pathogenic properties in psoriasis. Aim of this study was to determine IL-36α expression in psoriatic arthritis (PsA) compared to rheumatoid (RA) and osteoarthritis (OA).
Methods: Synovial tissue gathered from arthritis patients were stained for IL-36α, IL-36 receptor (IL-36R) and IL-36R antagonist (IL-36Ra) by immunohistochemistry and immunofluorescence. Lysates were tested for IL-36α by western blot analysis. Synovial fibroblasts (FLS) stimulated with IL-36α were assessed for cytokine expression.
Results: The IL-36R and its ligands IL-36α and IL-36Ra could be detected in inflammatory arthritis in the synovial lining layer as well as cellular infiltrates. IL-36α was significantly higher expressed in PsA and RA synovium compared to OA (p=0.0011 and p<0.0001, respectively). No differences were seen in IL-36R and IL-36Ra. The expression of IL-36α was confirmed by western blot analysis. IL-36α induced expression of IL-6 and IL-8 in FLS. CD138-positive plasma cells were determined as major cellular source for IL-36α.
Conclusion: We described that the novel cytokine IL-36α is upregulated in PsA and RA synovium mainly expressed by plasma cells. This insight needs further studies to determine if the IL-36 family can function as a potential target for arthritis therapy.
M. E. Messbacher,
D. L. Baeten,
G. A. Schett,
A. J. Hueber,
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ACR Meeting Abstracts - https://acrabstracts.org/abstract/plasma-cells-express-the-novel-cytokine-interleukin-36%ce%b1-in-psoriatic-and-rheumatoid-arthritis-synovium/