ACR Meeting Abstracts

ACR Meeting Abstracts

Abstract Number: 541

Microrna-125b Expression in PBMCs Is Associated with Disease Activity in Patients with Early Rheumatoid Arthritis

Veronika Hruskova1,2, Romana Jandová3, Lucia Vernerova4, Herman F Mann5, Klára Prajzlerová4, Maria Filkova4, Karel Pavelka5, Jiri Vencovsky5 and Ladislav Senolt5, 1Institute of Rheumatology and Department of Rheumatology; Faculty of Science Charles University, Prague, Czech Republic, 2Faculty of Science, Charles University, Prague, Czech Republic, 3Institute of Rheumatology and Department of Rheumatology, Prague, Czech Republic, 4Institute of Rheumatology and Department of Rheumatology, 1st Faculty of Medicine, Charles University, Prague, Czech Republic, 5Institute of Rheumatology and Department of Rheumatology, 1st Faculty of Medicine, Charles University, Prague, Czech Republic, Prague, Czech Republic

Meeting: 2015 ACR/ARHP Annual Meeting

Date of first publication: September 29, 2015

Keywords: , ,

Session Information

Date: Sunday, November 8, 2015

Title: Rheumatoid Arthritis - Human Etiology and Pathogenesis Poster I

Session Type: ACR Poster Session A

Session Time: 9:00AM-11:00AM

Background/Purpose: MicroRNAs (miRNAs) are small non-coding RNAs that function as negative regulators of gene expression at posttranscription level and play a significant role in rheumatoid arthritis (RA) (1). The expression of TNF-α, a key pro-inflammatory cytokine of RA pathogenesis, is negatively regulated by miR-125b (2). Therefore, the aim of this study was to evaluate the expression of miRNA-125b in peripheral blood mononuclear cells (PBMCs) and its association with disease activity and achievement of remission in early RA patients. 

Methods: A total of 60 patients (44 females; mean age 54.1 years) with early rheumatoid arthritis were studied. Total RNA was isolated from PBMCs collected from patients before and three months after the start of glucocorticoids and disease modifying antirheumatic drugs. The expression of miRNA-125b was determined by quantitative PCR. Small nucleolar RNA RNU44 was used for normalization. Relative expression was calculated as 2DCt. Disease activity of RA patients was assessed according to the 28-Joint Count Disease Activity Score (DAS28), the Simplified Disease Activity Index (SDAI) and Clinical Disease Activity Index (CDAI).  

Results: We found an inverse association between miRNA-125b expression and disease activity as assessed by DAS28 (r= -0.404; p= 0.001), SDAI (r= -0.334; p= 0.009) and CDAI (r= -0.263; p= 0.043) at baseline. The expression of miRNA-125b was significantly up-regulated three months after the start of treatment in all RA patients (p=0.005). The up-regulation of miRNA-125b after the three months of treatment was particularly observed in patients who achieved DAS28 defined remission compared to those who did not achieve the treatment target after 12 months.  Age, sex, autoantibodies (RF-IgM, anti-CCP) and smoking did not affect miRNA-125b expression in PBMC. 

Conclusion: The up-regulation of miRNA-125b in PMBCs following treatment initiation may represent potential biomarker predicting achievement of remission in early RA patients.

Acknowledgement: IGA project No NT 14498

Literature:

  1. Duroux-Richard I, Jorgensen C, Apparailly F. What do microRNAs mean for rheumatoid arthritis? Arthritis Rheumatism. 2012 Jan; 64(1):11-20
  2. Tili E, Michaille JJ, Cimino A, Costinean S, Dumitru CD, Adair B, Fabbri M, Alder H, Liu CG, Calin GA, Croce CM. Modulation of miR-155 and miR-125b levels following lipopolysaccharide/TNF-alpha stimulation and their possible roles in regulating the response to endotoxin shock. Journal of Immunol. 2007 Oct 15; 179(8):5082-9.
Disclosure: V. Hruskova, None; R. Jandová, None; L. Vernerova, None; H. F. Mann, None; K. Prajzlerová, None; M. Filkova, None; K. Pavelka, None; J. Vencovsky, None; L. Senolt, None.

To cite this abstract in AMA style:

Hruskova V, Jandová R, Vernerova L, Mann HF, Prajzlerová K, Filkova M, Pavelka K, Vencovsky J, Senolt L. Microrna-125b Expression in PBMCs Is Associated with Disease Activity in Patients with Early Rheumatoid Arthritis [abstract]. Arthritis Rheumatol. 2015; 67 (suppl 10). https://acrabstracts.org/abstract/microrna-125b-expression-in-pbmcs-is-associated-with-disease-activity-in-patients-with-early-rheumatoid-arthritis/. Accessed .

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