Measurement of the Pro-Coagulant Activity of Microparticles in Patients with Inflammatory Rheumatic Diseases: Prospective Study

Christine Mekhail¹, Xavier Guillot², Clément Prati³, Philippe Saas⁴, Guillaume Mourey⁵ and Daniel Wendling⁶, ¹Rheumatology, CHRU, Besançon, France, ²PEPITE EA 4267, Bourgogne-Franche-Comté university, Besançon, France, ³Rheumatology, University Hospital - Bourgogne Franche Comté University, Besancon, France, ⁴INSERM UMR1098, Besançon university hospital, Besançon, France, ⁵EFS, Besançon, France, ⁶Rheumatology, University Hospital - Bourgogne Franche Comté University, Besançon, France

Meeting: 2018 ACR/ARHP Annual Meeting

Keywords: Arthritis and microparticles

Session Information

Date: Tuesday, October 23, 2018

Title: Miscellaneous Rheumatic and Inflammatory Diseases Poster III: Sarcoid, Inflammatory Eye Disease, and Autoinflammatory Disease

Session Type: ACR Poster Session C

Session Time: 9:00AM-11:00AM

Background/Purpose:

Microparticles (MPs) are small membrane-bound vesicles that arise from activated and dying cells. Although the majority of MPs in the blood originate from platelets, all cells appear to be able to release MPs. Many studies have raised the implication of these MP in various processes: inflammation, thrombosis, angiogenesis. Previous studies reported inconsistent results in inflammatory rheumatic diseases. Studies have shown the correlation between the circulating MPs, platelet MPs and lymphocyte MPs in patients with spondyloarthritis (SpA) compared to control patients. For rheumatoid arthritis (RA), platelet MPs levels were correlated with DAS28.

The aim of this study was to search a possible correlation between the disease activity and the pro-coagulant property of microparticles, potential indirect marker of inflammation.

Methods:

The test used (STA Procoag PPL®) is a standardised automated test. Results are expressed as coagulation times (in seconds). It is a functional test that provides information on the procoagulant potential of microparticles. The microparticles supply the phospholipids expressed on their membrane surface and the test provides calcium and factor Xa necessary to initiate coagulation : the shorter the coagulation time the greater the procoagulant activity of the phospholipids being studied, suggesting a higher number of MP.

This is a prospective, single-center study, including 39 patients with spondyloarthritis (ASAS criteria), 37 with rheumatoid arthritis (ACR criteria) and 26 control patients (healthy subjects, osteoarthritis). All patients underwent STA Procoag PPL test, and we collected medical data : disease activity (BASDAI, BASFI, DAS28vs and DAS28crp and HAQ), biological inflammation (VS, CRP), duration of disease, and current treatment.

Results:

The in vitro clotting time of serum of patients with spondyloarthritis and rheumatoid arthritis compared with controls was not significantly different (p = 0.23 and p = 0.44, respectively). Regarding the activity scores of inflammatory rheumatic disease: BASDAI and BASFI, DAS28esr, DAS28crp and HAQ for patients with RA, no correlation between these data and coagulation time was found; the same goes for biological inflammation (ESR, CRP), duration and type of treatment (Nonsteroidal anti-inflammatory drugs, DMARDs, biologics).

STA PPL	Controls (n=26)		SpA (n=39)		p	RA (n=37)		p
Mean ± SD	62,70 ± 11,58		66,48 ±12,77		p = 0,23	65,25±13,54		P = 0,44
BASDAI		r=0,0602 p=0,72		DAS28 esr			r= 0,230 p=0,17
BASFI		r=-0,125 p=0,45		DAS28 crp			r=0,151 p=0,37

Conclusion:

In this study, there is neither difference in values of procoagulant activity of MPs between inflammatory rheumatic diseases and control subjects, nor correlation with their activity scores or biological inflammation.

Disclosure: C. Mekhail, None; X. Guillot, None; C. Prati, None; P. Saas, None; G. Mourey, None; D. Wendling, None.

To cite this abstract in AMA style:

Mekhail C, Guillot X, Prati C, Saas P, Mourey G, Wendling D. Measurement of the Pro-Coagulant Activity of Microparticles in Patients with Inflammatory Rheumatic Diseases: Prospective Study [abstract]. Arthritis Rheumatol. 2018; 70 (suppl 9). https://acrabstracts.org/abstract/measurement-of-the-pro-coagulant-activity-of-microparticles-in-patients-with-inflammatory-rheumatic-diseases-prospective-study/. Accessed .

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