ACR Meeting Abstracts

ACR Meeting Abstracts

  • Meetings
    • ACR Convergence 2024
    • ACR Convergence 2023
    • 2023 ACR/ARP PRSYM
    • ACR Convergence 2022
    • ACR Convergence 2021
    • ACR Convergence 2020
    • 2020 ACR/ARP PRSYM
    • 2019 ACR/ARP Annual Meeting
    • 2018-2009 Meetings
    • Download Abstracts
  • Keyword Index
  • Advanced Search
  • Your Favorites
    • Favorites
    • Login
    • View and print all favorites
    • Clear all your favorites
  • ACR Meetings

Abstract Number: 2494

Incidence and Severity of Spondyloarthritis and Crohn’s Ileitis Are Determined by Interaction Between the Microbiota and Genetic Susceptibility in Beta-Glucan-Treated SKG Mice

Ranjeny Thomas1, Linda Rehaume1, Daniel Aguirre de Cárcer2, Stan Mondot2, Jared Velasco1, Helen Benham1, Merja Ruutu1, Mark Morrison2 and Michael McGuckin3, 1University of Queensland Diamantina Institute, Brisbane, Australia, 2CSIRO Livestock Industries, Brisbane, Australia, 3Mater Medical Research Institute, Brisbane, Australia

Meeting: 2012 ACR/ARHP Annual Meeting

Keywords: Spondylarthropathy

  • Tweet
  • Click to email a link to a friend (Opens in new window) Email
  • Click to print (Opens in new window) Print
Session Information

Title: Spondyloarthritis and Psoriatic Arthritis - Pathogenesis, Etiology

Session Type: Abstract Submissions (ACR)

Background/Purpose:

Spondyloarthritis and inflammatory bowel disease (IBD) or microscopic gut inflammation co-exist in many patients. These conditions share genetic associations in the IL23R signaling pathway. Rodent models of spondyloarthritis or IBD typically improve in germ free (GF) conditions and intestinal microbial diversity is reduced in Crohn’s disease, with enrichment in Gram negative rods. Thus resident microbiota are implicated in pathogenesis. We hypothesized that microbiota directly affect incidence and severity of spondyloarthritis and ileitis in SKG mice, where the ZAP-70W163Cmutation of the BALB/c strain reduces T cell receptor signaling.

Methods:

SKG and BALB/c mice were housed in SPF or rederived to GF conditions then injected i.p. with 1,3-D beta-glucan (curdlan). Paw width was scored for 8 weeks. Joint, tail and small intestinal histological sections were scored at sacrifice. The fecal microbiota of BALB/c and SKG mice was assessed by 454 pyrosequencing 0, 3, 7, 10 and 14 days after curdlan treatment. Double principal components analysis (DPCA) and other methods were used to compare phylogenetic groups across samples.

Results:

In SPF conditions, i.p. curdlan triggered IL-23-dependent severe spondyloarthritis and Crohn’s-like ileitis in 100% and 70% of female SKG mice respectively. BALB/c control mice developed mild peripheral arthritis without ileitis. In GF conditions, the incidence of spondyloarthritis in SKG mice was 10% and ileitis 0%. No priming of anti-proteoglycan autoantibodies occurred without spondyloarthritis. Spondyloarthritis and ileitis incidence and severity, and anti-proteoglycan antibodies were restored when GF mice were reconstituted with a limited bacterial consortium. Within days after curdlan under SPF but not GF conditions, IL-23 and Grp-78 mRNA increased in the ileum, and activated dendritic cells (DC) were recruited to the mesenteric lymph nodes, where IL-17 mRNA expression increased. By DPCA of fecal and cecal microbiota, a large shift in community structure occurred in SKG but not BALB/c mice 3 days after curdlan, related to increased Bacteroides-affiliated sequences. Segmented filamentous bacteria were absent. When BALB/c and SKG mice were cohoused after weaning and injected with curdlan at 6 weeks of age, arthritis and ileitis severity was similar in BALB/c and SKG mice cohoused in each cage.

Conclusion:

Microbiota are necessary and sufficient for priming spondyloarthritis and Crohn’s-like ileitis by DC in gut draining lymph nodes in SKG mice. The SKG mutation predisposes to lack of control of particular transmissable microbial species triggered by systemic delivery of curdlan, which enhance susceptibility to spondyloarthritis and Crohn’s-like ileitis.


Disclosure:

R. Thomas,
None;

L. Rehaume,
None;

D. Aguirre de Cárcer,
None;

S. Mondot,
None;

J. Velasco,
None;

H. Benham,
None;

M. Ruutu,
None;

M. Morrison,
None;

M. McGuckin,
None.

  • Tweet
  • Click to email a link to a friend (Opens in new window) Email
  • Click to print (Opens in new window) Print

« Back to 2012 ACR/ARHP Annual Meeting

ACR Meeting Abstracts - https://acrabstracts.org/abstract/incidence-and-severity-of-spondyloarthritis-and-crohns-ileitis-are-determined-by-interaction-between-the-microbiota-and-genetic-susceptibility-in-beta-glucan-treated-skg-mice/

Advanced Search

Your Favorites

You can save and print a list of your favorite abstracts during your browser session by clicking the “Favorite” button at the bottom of any abstract. View your favorites »

All abstracts accepted to ACR Convergence are under media embargo once the ACR has notified presenters of their abstract’s acceptance. They may be presented at other meetings or published as manuscripts after this time but should not be discussed in non-scholarly venues or outlets. The following embargo policies are strictly enforced by the ACR.

Accepted abstracts are made available to the public online in advance of the meeting and are published in a special online supplement of our scientific journal, Arthritis & Rheumatology. Information contained in those abstracts may not be released until the abstracts appear online. In an exception to the media embargo, academic institutions, private organizations, and companies with products whose value may be influenced by information contained in an abstract may issue a press release to coincide with the availability of an ACR abstract on the ACR website. However, the ACR continues to require that information that goes beyond that contained in the abstract (e.g., discussion of the abstract done as part of editorial news coverage) is under media embargo until 10:00 AM ET on November 14, 2024. Journalists with access to embargoed information cannot release articles or editorial news coverage before this time. Editorial news coverage is considered original articles/videos developed by employed journalists to report facts, commentary, and subject matter expert quotes in a narrative form using a variety of sources (e.g., research, announcements, press releases, events, etc.).

Violation of this policy may result in the abstract being withdrawn from the meeting and other measures deemed appropriate. Authors are responsible for notifying colleagues, institutions, communications firms, and all other stakeholders related to the development or promotion of the abstract about this policy. If you have questions about the ACR abstract embargo policy, please contact ACR abstracts staff at [email protected].

Wiley

  • Online Journal
  • Privacy Policy
  • Permissions Policies
  • Cookie Preferences

© Copyright 2025 American College of Rheumatology