ACR Meeting Abstracts

ACR Meeting Abstracts

Abstract Number: 1944

Expression of Human Leukocyte Antigen-G in Polymyositis or Dermatomyositis

Xiaolan Tian1, Xiaoming Shu2, Xin Lu1, Qinglin Peng3 and Guochun Wang4, 1Department of rheumatology, China-Japan Friendship Hospital, Beijing, China, 2Rheumatology, China-Japan Friendship Hospital, Beijing, China, 3Department of Rheumatology,, China-Japan Friendship Hospital, Beijing, China, 4Department of Rheumatology, China-Japan Friendship Hospital, Beijing, China

Meeting: 2012 ACR/ARHP Annual Meeting

Keywords:

Session Information

Title: Muscle Biology, Myositis and Myopathies: Genetics, Autoantibodies and other Molecular Aspects of Idiopathic Inflammatory Myopathies and Models

Session Type: Abstract Submissions (ACR)

Background/Purpose: Human leukocyte antigen-G is a non-classical MHC–I molecule that may be involved in the pathogenesis of autoimmune diseases, but the significance of HLA-G in polymyositis/dermatomyositis (PM/DM) remains to be determined. The aim of this study was to determine HLA-G expression in patients with PM/DM.

Methods: Consecutive cryosections of muscle biopsies obtained from 30 PM/DM patients(22 DM, 8 PM)and 8 healthy controls were detected by immunohistochemical analysis. Serum soluble HLA-G (sHLA-G) levels of 96 patients (26 PM, 70 DM) and 35 matched healthy controls were measured by ELISA. The relationship between sHLA-G levels and clinical or laboratory variables in PM/DM patients was analyzed.

Results:  HLA-G had a higher expression in 18 of 30 cases of PM/DM (60%) than that in 0 of 8 healthy controls (P =0.000). Serum level of sHLA-G in PM/DM patients were significantly higher than healthy controls [(43.96±70.20)U/ml vs (4.22±5.13)U/ml, P=0.000]. Importantly, serum sHLA-G levels in non-treated PM/DM patients(75.75±99.07 U/ml)were significantly higher than that in treated patients(33.94±51.97)U/ml, P=0.021]. Spearman rank correlation analysis showed serum sHLA-G levels were negatively correlated with CD3+Tcells(r=-0.233, P=0.047)and CD4+Tcells (r=-0.287, P=0.015) in peripheral blood in patients with PM/DM.

Conclusion: HLA-G was found to be highly expressed in muscle tissues and sera from patients with PM/DM. Serum sHLA-G is increased in PM/DM patients and is associated with lower level of CD3+T cells and CD4+T cells. These data support the notion that HLA-G overexpression has a role in the progression of PM/DM through T cells.

Disclosure:

X. Tian,
None;

X. Shu,
None;

X. Lu,
None;

Q. Peng,
None;

G. Wang,
None.

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