Comprehensive Analysis of Protein Expression in Peripheral Blood Mononuclear Cells From Patients with Behcet’s Disease

Takuya Yoshioka¹, Manae Kurokawa², Yukiko Takakuwa¹, Hiromasa Nakano¹, Seido Ooka³, Nobuko Iizuka², Toshiyuki Sato², Mitsumi Arito², Kouhei Nagai⁴, Kazuki Okamoto², Naoya Suematsu², Noboru Suzuki⁵, Shoichi Ozaki⁶ and Tomohiro Kato², ¹Division of Rheumatology and Allergy, Department of Internal Medicine, St. Marianna University School of Medicine, Kawasaki, Japan, ²Dept of Biochemistry, St. Marianna Univ School Med, Kawasaki, Japan, ³St. Marianna University School of Medicine, Kawasaki, Japan, ⁴Kinki Univ., Kinokawa, Japan, ⁵Department of Immunology and Medicine, St. Marianna University School of Medicine, Kawasaki, Japan, ⁶Internal Medicine, St. Marianna University School of Medicine, Kawasaki, Japan

Meeting: 2012 ACR/ARHP Annual Meeting

Keywords: Behcet's syndrome and proteomics

Session Information

Title: Miscellaneous Rheumatic and Inflammatory Diseases: Periodic Fever Syndromes

Session Type: Abstract Submissions (ACR)

Background/Purpose: To elucidate the pathophysiology of Behcet’s disease (BD) and to establish biomarkers for the disease, we comprehensively analyzed protein expression of peripheral blood mononuclear cells (PBMCs).

Methods: PBMCs were obtained from 16 patients with BD, 16 patients with rheumatoid arthritis (RA), and 16 healthy control (HC) subjects. Proteins, extracted from the PBMCs, were separated by 2-dimensional differential gel electrophoresis. Proteins were identified by mass spectrometry.

Results: As a result, a total of 563 protein spots were detected. Compared to HC, 14 spots showed significantly higher intensity with more than 1.2 folds, and 9 spots showed significantly lower intensity with less than 1/1.2 folds in BD. The spots with higher intensity included proteins involved in T cell activation, metabolism of pre-mRNAs, and protein trafficking. The spots with lower intensity included cytoskeltal proteins. Similarly, compared to RA, 98 spots showed significantly higher intensity with more than 1.2 folds, and 17 spots showed significantly lower intensity with less than 1/1.2 folds in BD. The spots with higher intensity in BD than in RA included proteins associated with bacteriolysis, oxidation/reduction, activation of transcription, regulation of kinases, and modulation of glycosylation. We completely discriminated the BD patients from the HC subjects and from the RA patients by multivariate analyses of 23 and 35 protein spots, respectively. The analyses using only 1-3 protein spots provided areas under the receiver operating characteristic curves of 0.797-0.898 in the discrimination of the BD patients from the HC subjects, from the RA patients, and from the both HC subjects and RA patients.

Conclusion: Taken together, the protein spots which contributed to the discrimination could be biomarker candidates for BD. In addition PBMC-derived proteins, expression of which was significantly altered in BD, may be involved in the pathophysiology of BD.

Disclosure:

T. Yoshioka,
None;

M. Kurokawa,
None;

Y. Takakuwa,
None;

H. Nakano,
None;

S. Ooka,
None;

N. Iizuka,
None;

T. Sato,
None;

M. Arito,
None;

K. Nagai,
None;

K. Okamoto,
None;

N. Suematsu,
None;

N. Suzuki,
None;

S. Ozaki,
None;

T. Kato,
None.

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