ACR Meeting Abstracts

ACR Meeting Abstracts

  • Meetings
    • ACR Convergence 2024
    • ACR Convergence 2023
    • 2023 ACR/ARP PRSYM
    • ACR Convergence 2022
    • ACR Convergence 2021
    • ACR Convergence 2020
    • 2020 ACR/ARP PRSYM
    • 2019 ACR/ARP Annual Meeting
    • 2018-2009 Meetings
    • Download Abstracts
  • Keyword Index
  • Advanced Search
  • Your Favorites
    • Favorites
    • Login
    • View and print all favorites
    • Clear all your favorites
  • ACR Meetings

Abstract Number: 1108

Antibody Repertoire Dynamics in Systemic Sclerosis after Myeloablative Autologous Hematopoietic Stem-Cell Transplantation or Cyclophosphamide Treatment

Julia Z. Adamska1,2, Leslie Crofford3, Daniel E. Furst4,5, Ellen Goldmuntz6, Lynette Keyes-Elstein7, Maureen D. Mayes8, Peter McSweeney9, Richard Nash9, Ashley Pinckney10, Beverly Welch11, Keith Sullivan12 and William H. Robinson1,2, 1Stanford University, Stanford, CA, 2VA Palo Alto Health Care System, Palo Alto, CA, 3Division of Rheumatology and Immunology, Vanderbilt University Medical Center, Nashville, TN, 4University of California Los Angeles, Los Angeles, CA, 5University of Washington, Seattle, WA, 6NIAID, NIH, Bethesda, MD, 7Clinical Statistics, Rho Federal Systems, Inc., Chapel Hill, NC, 8Rheumatology, University of Texas McGovern Medical School, Houston, TX, 9Colorado Blood Cancer Institute, Denver, CO, 10Rho Federal Systems, Inc., Chapel Hill, NC, 11National Institutes of Health, Bethesda, MD, 12Duke University Medical Center, Durham, NC

Meeting: 2018 ACR/ARHP Annual Meeting

Keywords: scleroderma and systemic sclerosis

  • Tweet
  • Click to email a link to a friend (Opens in new window) Email
  • Click to print (Opens in new window) Print
Session Information

Date: Monday, October 22, 2018

Title: Systemic Sclerosis and Related Disorders – Basic Science Poster II

Session Type: ACR Poster Session B

Session Time: 9:00AM-11:00AM

Background/Purpose: Myeloablative autologous hematopoietic stem-cell transplantation (HSCT) was recently demonstrated to provide benefit over monthly cyclophosphamide (CYC) in the treatment of diffuse cutaneous systemic sclerosis (dcSSc) (1).  As the dysregulation of the B cell compartment is implicated in the pathogenesis of SSc (2), we used antibody repertoire sequencing to characterize B cell repertoire dynamics over the course of HSCT and CYC treatment with the goal of identifying characteristics that differentiate responders from non-responders.

Methods: Immunoglobulin heavy-chain (IGH) sequencing from peripheral blood RNA was performed on HiSeq 2500 using an approach adapted from previously described methods (3). Fastq files de-multiplexed by sample were processed with MIGEC and MIXCR software to establish consensus sequences and identify unique clonotypes within each patient’s repertoire. Further analysis was performed using VDJtools, Immcantation, and IMGT-HighVQuest software. Samples yielding fewer than 3,500 sequences aligned to IGH genes were excluded from analysis. Event-free survival (EFS) at 54 months post treatment, characterized by no significant organ damage, was used to define treatment responders. The cohort included 13 HSCT recipients (8 responders), 14 CYC treated patients (7 responders) and 15 healthy controls. Responders were assessed at 3-4 time points (baseline and at 26 and/or 36 and/or 48 months post-treatment), non-responders at 2-3 time points (baseline and any time points leading up to and including EFS failure) and healthy controls at a single time point.

Results: Mean IGH repertoire diversity as assessed by the Inverse Simpson Diversity Index was equivalent in both dcSSc treatment groups and healthy controls at baseline (HSCT 1037±604.7, CYC 919±735.5, and healthy controls 1232±600.6). At last study time point, HSCT recipient responders exhibit an increase in mean IGH repertoire diversity compared to baseline (1100±578.9 vs. 1638±420.5, p=0.037, paired t-test) which was not observed in HSCT non-responders. Although in comparing last study point to baseline, there was no difference observed in CYC treated patients, CYC treated non-responders exhibit lower mean IGH repertoire diversity at last study time point than healthy controls (1232±600.6 vs. 551.2±365, p=0.012, unpaired t-test).

 

Conclusion: Increased mean IGH repertoire diversity from baseline is seen in HSCT responders.  CYC treated non-responders have decreased mean IGH repertoire diversity as compared to healthy controls.

References: (1) Sullivan KM et al. N Engl J Med 2018;378:35-47. (2) Yoshizaki A. Immunol Lett 2018;195:76-82. (3) Turchaninova MA et al. Nat Prot 2016;11:1599-1616.


Disclosure: J. Z. Adamska, None; L. Crofford, None; D. E. Furst, None; E. Goldmuntz, None; L. Keyes-Elstein, None; M. D. Mayes, None; P. McSweeney, None; R. Nash, None; A. Pinckney, None; B. Welch, None; K. Sullivan, None; W. H. Robinson, None.

To cite this abstract in AMA style:

Adamska JZ, Crofford L, Furst DE, Goldmuntz E, Keyes-Elstein L, Mayes MD, McSweeney P, Nash R, Pinckney A, Welch B, Sullivan K, Robinson WH. Antibody Repertoire Dynamics in Systemic Sclerosis after Myeloablative Autologous Hematopoietic Stem-Cell Transplantation or Cyclophosphamide Treatment [abstract]. Arthritis Rheumatol. 2018; 70 (suppl 9). https://acrabstracts.org/abstract/antibody-repertoire-dynamics-in-systemic-sclerosis-after-myeloablative-autologous-hematopoietic-stem-cell-transplantation-or-cyclophosphamide-treatment/. Accessed .
  • Tweet
  • Click to email a link to a friend (Opens in new window) Email
  • Click to print (Opens in new window) Print

« Back to 2018 ACR/ARHP Annual Meeting

ACR Meeting Abstracts - https://acrabstracts.org/abstract/antibody-repertoire-dynamics-in-systemic-sclerosis-after-myeloablative-autologous-hematopoietic-stem-cell-transplantation-or-cyclophosphamide-treatment/

Advanced Search

Your Favorites

You can save and print a list of your favorite abstracts during your browser session by clicking the “Favorite” button at the bottom of any abstract. View your favorites »

All abstracts accepted to ACR Convergence are under media embargo once the ACR has notified presenters of their abstract’s acceptance. They may be presented at other meetings or published as manuscripts after this time but should not be discussed in non-scholarly venues or outlets. The following embargo policies are strictly enforced by the ACR.

Accepted abstracts are made available to the public online in advance of the meeting and are published in a special online supplement of our scientific journal, Arthritis & Rheumatology. Information contained in those abstracts may not be released until the abstracts appear online. In an exception to the media embargo, academic institutions, private organizations, and companies with products whose value may be influenced by information contained in an abstract may issue a press release to coincide with the availability of an ACR abstract on the ACR website. However, the ACR continues to require that information that goes beyond that contained in the abstract (e.g., discussion of the abstract done as part of editorial news coverage) is under media embargo until 10:00 AM ET on November 14, 2024. Journalists with access to embargoed information cannot release articles or editorial news coverage before this time. Editorial news coverage is considered original articles/videos developed by employed journalists to report facts, commentary, and subject matter expert quotes in a narrative form using a variety of sources (e.g., research, announcements, press releases, events, etc.).

Violation of this policy may result in the abstract being withdrawn from the meeting and other measures deemed appropriate. Authors are responsible for notifying colleagues, institutions, communications firms, and all other stakeholders related to the development or promotion of the abstract about this policy. If you have questions about the ACR abstract embargo policy, please contact ACR abstracts staff at [email protected].

Wiley

  • Online Journal
  • Privacy Policy
  • Permissions Policies
  • Cookie Preferences

© Copyright 2025 American College of Rheumatology