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Abstract Number: 136

14-3-3π(eta) Protein in Juvenile Idiopathic Arthritis

Austin Dalrymple1,2,3, Paul Tuttle IV4, Lance Feller5, Olga S. Zhukov6, Robert J. Lagier7, Robert Bridgforth8, Gary J Williams9, Joanna Popov10, Stanley J. Naides6 and Terry Moore11, 1Division of Rheumatology and Pediatric Rheumatology, Saint Louis University School of Medicine, St Louis, MO, 2Division of Adult and Pediatric Rheumatology, Saint Louis University, St Louis, MO, 3Division of Rheumatology and Pediatric Rheumatology, Saint Louis University, St Louis, MO, 4Saint Louis University, Saint Louis, MO, 5Former fellow, Saint Louis University, Watertown, ME, 6Immunology, Quest Diagnostics Nichols Institute, San Juan Capistrano, CA, 7Research Support, Alameda, Quest Diagnostics Alameda, Alameda, CA, 8nichols Institute, Quest Diagnostics, San Juan Capistrano, CA, 9Nicolas Institue, Quest Diagnostics, San Juan Capistrano, CA, 10Immunology, Quest Diagnostics, San Jian Capistrano, CA, 11Rheumatology, Saint Louis University, St. Louis, MO

Meeting: 2017 Pediatric Rheumatology Symposium

Keywords: Biomarkers and juvenile idiopathic arthritis (JIA)

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Session Information

Date: Thursday, May 18, 2017

Session Title: Genetics and Pathogenesis Poster Session

Session Type: Abstract Submissions

Session Time: 5:30PM-7:00PM

Background/Purpose: 14-3-3 proteins are chaperonins found in all eukaryotic cells. There are multiple isoforms which are thought to be involved in intracellular signaling and transcription regulation. Recent work has implicated the η (eta) isoform as having diagnostic potential in inflammatory arthritides. Its utility in JIA has not been established. Our preliminary prior investigation indicated positivity in some JIA patients. In this study, we investigated a much larger cohort of patients with JIA and disease and healthy controls.

Methods: Measurement of 14-3-3η protein was evaluated in 29 rheumatoid factor (RF) positive (pos) polyarticular (poly) JIA patients, 29 RF negative (neg) poly patients, 34 oligoarticular (oligo) patients, 12 systemic-onset (SO) patients, 19 adult rheumatoid arthritis (RA) patients, 60 patients with systemic lupus (SLE), and 20 healthy controls by the assay established at Quest Diagnostics. Comparisons were made to CBC, ESR, CRP, RF and anti-CCP isotypes, and ANA positivity.

Results: 14-3-3η at 0.2 ng/ml or higher was considered positive; values of 0.5 ng/mL or greater have been considered prognostic of poor outcome in adults. Ten of 29 (34%) RF pos polys were positive for the 14-3-3η protein; 8 (28%) had values > 0.5 ng/mL. Nine of 29 (31%) RF neg polys were positive; 8/29 (28%) had values >0.5 ng/mL. Only 6/34 (18%) oligos were positive; 5/34 (15%) >0.5 ng/mL. Only 2/12 (16%) SO were positive; 1/12 (8%) >0.5 ng/mL. In the disease controls, 14/60 (23%) SLE were positive, but only 7/60 (12%) >0.5 ng/mL. 7/19 RA patients were positive, 4/19 (21%) >0.5 ng/mL. In the healthy controls, only 3/20 (15%) were positive, 1/20 (5%) > 0.5 ng/mL. The RF pos and RF neg polys positivity, especially at values >0.5 ng/mL compared favorably with the adult RA patient and were significant compared to disease and healthy controls. A weak correlation was noted between 14-3-3η positivity and CRP. Five of the 8 RF neg polys at original diagnosis that were 14-3-3η positive at >0.5 ng/mL have subsequently developed a positive RF and an anti-CCP antibody isotype. Also, the one SO positive for 14-3-3η >0.5 ng/mL also developed a positive RF.

Conclusion:  Significant levels of 14-3-3η protein can be found in about 30% of RF pos and RF neg poly JIA patients. It may represent a new biomarker for RF neg poly JIA patients and a marker indicating the possibility of these patients becoming RF/anti-CCP antibody positive in the future. Further longitudinal studies are required to confirm these findings.


Disclosure: A. Dalrymple, None; P. Tuttle IV, None; L. Feller, None; O. S. Zhukov, 3; R. J. Lagier, 3; R. Bridgforth, None; G. J. Williams, 3; J. Popov, None; S. J. Naides, 3,1; T. Moore, None.

To cite this abstract in AMA style:

Dalrymple A, Tuttle P IV, Feller L, Zhukov OS, Lagier RJ, Bridgforth R, Williams GJ, Popov J, Naides SJ, Moore T. 14-3-3π(eta) Protein in Juvenile Idiopathic Arthritis [abstract]. Arthritis Rheumatol. 2017; 69 (suppl 4). https://acrabstracts.org/abstract/14-3-3%cf%80eta-protein-in-juvenile-idiopathic-arthritis/. Accessed January 16, 2021.
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