Session Information
Session Type: Abstract Submissions (ACR)
Background/Purpose: The immunoregulatory function of interleukin (IL)-29 has recently been recognized. However, little is known about the involvement of IL-29 in the pathogenesis of rheumatoid arthritis (RA). We here investigated the potential role of IL-29 in the synovial inflammation of RA.
Methods: The transcript levels of IL-29 and its specific receptor IL-28Rα in peripheral blood mononuclear cells (PBMC) and synovium were determined by real-time reverse transcription-polymerase chain reaction (real-time PCR). The concentrations of IL-29 in serum and synovial fluid (SF) were quantified by enzyme-linked immunoassay (ELISA), and the correlation of serum IL-29 levels with disease activity in RA patients was investigated. Furthermore, the expression of IL-29 in RA synovium was examined by immunohistochemistry and double immunofluorescence analysis. Finally, the expression of IL-6, IL-8, IL-10, IL-17 and matrix-metalloproteinase-3 (MMP-3) in synovial fibroblasts upon IL-29 stimulation was determined by real-time PCR.
Results: IL-29 and IL-28Rα mRNA expression in PBMC were significantly increased in patients with RA compared with healthy controls (HC). The serum levels of circulating IL-29 were higher in RA than those in HC. RA SF also conferred the increased IL-29 levels compared to osteoarthritis (OA) SF. However, serum IL-29 levels showed no significant correlation with the RA disease activity. IL-29 was markedly elevated in RA synovium compared to tissues from HC, and was mostly expressed in the lining region of synovium. Moreover, IL-29 was expressed predominately in synovial macrophages and fibroblasts. RA synovial fibroblasts exposed to IL-29 specifically upregulated IL-6, -8 and MMP-3, downregualted IL-10, but showed no effect on IL-17.
Conclusion: The findings in the present study indicate, for the first time, that IL-29 is dysregulated in patients with RA, which may contribute to the RA pathogenesis via inducing the production of proinflammatory cytokines, chemokines or matrix-metalloproteinases in synovial fibroblasts.
Disclosure:
M. Zhang,
the National Natural Science Foundation of China (No. 30901332, 81172845),
2;
F. Wang,
the National Natural Science Foundation of China (No. 30901332, 81172845),
2;
L. Xu,
the National Natural Science Foundation of China (No. 30901332, 81172845),
2;
W. Tan,
the National Natural Science Foundation of China (No. 30901332, 81172845),
2.
« Back to 2012 ACR/ARHP Annual Meeting
ACR Meeting Abstracts - https://acrabstracts.org/abstract/interleukin-29-modulates-proinflammatory-cytokine-production-in-synovial-inflammation-of-rheumatoid-arthritis/