ACR Meeting Abstracts

ACR Meeting Abstracts

Abstract Number: 572

Immunization With Hepatitis B Vaccine Accelerates SLE-Like Disease In An Animal Model

Nancy Agmon-Levin1, María Teresa Arango2, Shaye Kivity3, Aviva Katzav4, Boris Gilburd5, Miri Blank6,7, Juan-Manuel Anaya8, Gisele Zandman-Goddard9, Joab Chapman10 and Yehuda Shoenfeld11, 1The Zabludowicz Center for Autoimmune Diseases, Tel-Hashomer, Israel, 2Doctoral Program in Biomedical Sciences Universidad del Rosario, Bogota, Colombia;Center for Autimmune Diseases Research – CREA, Universidad del Rosario,, Bogota, Colombia, 3Zabludowicz Center for Autoimmune Diseases, Sheba Medical Center, affliated to Sackler Faculty of Medicine, Tel-Aviv University Israel, Tel-Aviv, Israel, 4Department of. Neurology and Sagol Neuroscience Center, Sheba Medical Center,Tel-Hashomer, Ramat Gan, Israel, 5The Zabludowicz Center for Autoimmune Diseases, Sheba Medical Center, Tel-Hashomer, Ramat-Gan, Israel, 6Zabludowicz Center for Autoimmune Diseases, Sheba Medical Center, Tel-Hashomer, affiliated to Sackler Medical School, Tel Aviv University, Ramat Gan, Israel, 7Zabludowicz Center for Autoimmune Diseases, Sheba Medical Center, affiliated to Sackler Faculty of Medicine,Tel Aviv University, Tel-Aviv, Tel-Aviv, Israel, 8School of Medicine and Health Sciences, Universidad del Rosario. Center for Autoimmune Diseases Research (CREA), Bogotá, Colombia, 9Internal Medicine C, Wolfson Medical Center;Sackler Faculty of Medicine, Tel-Aviv University, Holon, Israel, 10Department of. Neurology and Sagol Neuroscience Center, Sheba Medical Center, Tel-Hashomer, Ramat Gan, Israel, 11The Zabludowicz Center for Autoimmune Diseases, Sheba Medical Center, Ramat Gan, Israel

Meeting: 2013 ACR/ARHP Annual Meeting

Keywords: ,

Session Information

Title: Systemic Lupus Erythematosus - Animal Models

Session Type: Abstract Submissions (ACR)

Background/Purpose:

Hepatitis-B vaccine (HBVv) was proven to be successful and cost effective in preventing HBV infection and associated liver disease. However, in recent years concerns were raised regarding its safety, particularly among patients with autoimmune diseases (i.e. SLE)#.  Additionally, the Aluminum adjuvant included in HBVv has been related to immune mediated adverse events.  Thus, in the current study we set to evaluate the effects of immunization with HBVv or Alum in an animal model of SLE.

Methods:

Three groups of 20 NZB/W F1 mice (Jackson Laboratory, Bar Harbor, ME), were immunized with HBVv (Engerix), Aluminum hydroxide (Alum) or phosphate buffered saline (PBS) at 8 and 12 weeks of age. Mice were followed for weight, autoantibodies titers, blood cell accounts, urine protein, kidney histology and neurocognitive functions (i.e. novel object recognition, staircase, Y-maze and the forced swimming tests).  

Results:

Mice immunized with HBVv had significantly higher titers of anti-dsDNA antibodies (p<0.01), accelerated proteinuria (p<0.05) and advanced kidney damage on biopsy compared to those immunized with PBS or Alum. Mice immunized with HBVv or Alum had decreased cells counts and hemoglobin levels (p<0.001) compare with mice immunized with PBS. Similarly, long and short term memory were significantly decreased following immunization with HBVv or Alum (p<0.01). Mice immunized with alum displayed anxiety-like behavior.

Conclusion:

Herein we have found that immunization with the HBVv accelerates kidney disease in an animal model of SLE-like disease. Both Alum and HBVv, which contains alum as an adjuvant, affected blood counts and neurocognitive functions. Further studies are required to elucidate the mechanisms by which different ingredient of HBVv affect SLE-like disease in animal models.

# Agmon-Levin N, et al. Ten cases of systemic lupus erythematosus related to hepatitis B vaccine. Lupus. 2009;18:1192-7.; Bassi N, et al. Induction of the ‘ASIA’ syndrome in NZB/NZWF1 mice after injection of complete Freund‘s adjuvant (CFA). Lupus. 2012;21:203-9.

Disclosure:

N. Agmon-Levin,
None;

M. Teresa Arango,
None;

S. Kivity,
None;

A. Katzav,
None;

B. Gilburd,
None;

M. Blank,
None;

J. M. Anaya,
None;

G. Zandman-Goddard,
None;

J. Chapman,
None;

Y. Shoenfeld,
None.

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