Cytokine and Chemokine Levels in Serum and Tears As Disease Activity Biomarkers in Patients with Primary Sjögren´s Syndrome

Angel Alejandro Castillo-Ortiz¹, Rosa Elda Barbosa-Cobos², Lizbeth Becerril-Mendoza³, Gustavo Lugo-Zamudio⁴, Virgilio Lima-Gómez⁴, Adan Moreno-Eutimio⁵, Nayeli Nieto-Velázquez⁵ and Angel Tzec-Pérez⁵, ¹Rheumatology, Hospital Juárez de México, Mérida, Mexico, ²Rheumatology, Hospital Juárez de México, Mexico, Mexico, ³Hospital Juárez de México, Mexico, Mexico, ⁴Hospital Juárez de México, México city, Mexico, ⁵Hospital Juárez de México, Mexico city, Mexico

Meeting: 2016 ACR/ARHP Annual Meeting

Date of first publication: September 28, 2016

Keywords: IL-1/IL-18, RANTES, Sjogren's syndrome, TNFSF13 and migration inhibitory factor (MIF)

Session Information

Date: Sunday, November 13, 2016

Title: Sjögren's Syndrome - Poster I: Translational Science

Session Type: ACR Poster Session A

Session Time: 9:00AM-11:00AM

Background/Purpose: Evidence suggests that levels of different cytokines in primary Sjögren´s syndrome (pSS) are associated with cell infiltration degree within lacrimal and salivary glands and severity of the disease. Chemokines appear to contribute to the pathogenesis of pSS. Purpose: To evaluate the association between serum and tear Th1/Th2/Th17 cytokine and chemokine levels and disease activity in patients with pSS.

Methods: Case-control study including 19 patients with pSS and 13 healthy controls. Disease activity was assessed by EULAR Sjögrens´s Syndrome Disease Activity Index (ESSDAI); serum and tear IFN-γ, TNF. IL-2, IL-4, IL-6, IL-10, IL-17, IP10, MCP-1, MIG, RANTES and IL-8 levels were measured by bead-based assays following the sandwich immunoassay principle (BD Cytometric Bead Array; BD Biosciences). Differences between groups were analyzed using the Mann-Whitney U test. Correlation was estimated by Spearman´s correlation coefficient.

Results: TNF, IL-2, IL-4, IL-6, IL-10, IP-10, MCP-1, RANTES and IL-8 tear levels were statistically different between controls and patients with pSS (p<0.005). A significant correlation (p>0.005) was shown in patients between serum IL-6 and serum IL-2 (r=0.652), IL-4 (r=0.731; serum IL-2 and serum IP-10 (r=0.640); serum IL-2 and tear IL-2 (r=0.614, IL-8 (r=0.723), IP-10 (r=0.795), MIG (r=0.755); serum IL-4 and tear IL-8 (r=0.696), MIG (r=0.707); serum IP-10 and serum MIG (r=0.847), RANTES (r=0.626); serum IP-10 and tear IP-10 (r=0.619); serum MIG and serum RANTES (r=0.719); serum MIG and tear IL-17 (r=0.619); serum RANTES and tear IL-2 (r=0.628), IL-4 (r=0.619), IL-6 (r=0.736), IL-8 (r=0.770), IP-10 (r=0.687), MCP-1 (r=0.566), MIG (r=0.740).

Conclusion: Increased tear Th1/th2 cytokine levels were found in pSS. No correlation was found between serum or tear cytokine and chemokine levels and pSS disease activity.

Disclosure: A. A. Castillo-Ortiz, None; R. E. Barbosa-Cobos, None; L. Becerril-Mendoza, None; G. Lugo-Zamudio, None; V. Lima-Gómez, None; A. Moreno-Eutimio, None; N. Nieto-Velázquez, None; A. Tzec-Pérez, None.

To cite this abstract in AMA style:

Castillo-Ortiz AA, Barbosa-Cobos RE, Becerril-Mendoza L, Lugo-Zamudio G, Lima-Gómez V, Moreno-Eutimio A, Nieto-Velázquez N, Tzec-Pérez A. Cytokine and Chemokine Levels in Serum and Tears As Disease Activity Biomarkers in Patients with Primary Sjögren´s Syndrome [abstract]. Arthritis Rheumatol. 2016; 68 (suppl 10). https://acrabstracts.org/abstract/cytokine-and-chemokine-levels-in-serum-and-tears-as-disease-activity-biomarkers-in-patients-with-primary-sjogrens-syndrome/. Accessed .

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