Session Type: Abstract Submissions (ACR)
To investigate a role of Adiponectin (AD) on the differentiation of naïve T cell to Th17 cell and bone erosion in collagen-induced arthritis (CIA) mice.
Purified naïve T cells were cultured in anti-CD3 mAb and anti-CD28 mAb precoated with TGF-β, IL-6, IL-23 in the presence and absence of AD (0.1, 1 or 10 μg/ml). After 72 hours of culture, the frequencies of Th17 cells were measured by flow cytometry; the expression of ROR-γt and IL-17 were determined by real-time PCR and ELISA. Intraarticularly injected of AD 10 μl (1 μg/1 μl) into the knee joint of CIA mice was to analysis the role of AD on CIA development and Th17 expression in vivo.
The frequencies of Th17 cells were significantly increased with the concentration of AD 10 μg/ml, but AD treatment with 0.1 and 1 μg/ml showed no obvious effect on the differentiation of naïve T cell to Th17 cell. The expression of IL-17 and ROR-γt mRNA were significantly increased oupon AD 0.1 μg/ml and 10 μg/ml AD stimulation. Consistently with mRNA expression, IL-17 levels were significantly elevated in culture supernatants after 10 μg/ml AD treatment. Intraarticular injection of AD into the knee joint of CIA mice aggravated arthritic development and bone erosion, triggered higher expression of IL-17 mRNA and its transcription factor including ROR-γt, IL-21, IL-22 and IL-23 in CIA model.
These findings identify a role of AD on enhancing the differenation of naïve T cell to Th17 cell, contributed to CIA bone erosion in CIA mice.
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ACR Meeting Abstracts - http://acrabstracts.org/abstract/adiponectin-promotes-the-differentiation-of-naive-t-cell-to-th17-cell-and-aggravates-collagen-induced-arthritis/